Monnet-Tschudi F, Eng L F, Matthieu J M, Honegger P
Institut de Physiologie, Université de Lausanne, Suisse.
Dev Neurosci. 1988;10(3):165-72. doi: 10.1159/000111966.
Serum-free aggregating cell cultures of fetal rat telencephalon were examined by a combined biochemical and double-labeling immunocytochemical study for the developmental expression of glial fibrillary acidic protein (GFAP) and glutamine synthetase (GS). It was found that these two astroglial markers are co-expressed at different developmental stages in vitro. During the phase of cellular maturation (i.e. between days 14 and 34), GFAP levels and GS activity increase rapidly and in parallel. At the same time, the number of immunoreactive cells increase while the long and thick processes staining in early cultures gradually disappear. The present results demonstrate that in this particular cell culture system only one type of astrocytes develops which expresses both GFAP and GS and which attains a relatively high degree of maturation.
通过生物化学和双标记免疫细胞化学相结合的研究方法，对胎鼠端脑无血清聚集细胞培养物进行检测，以研究胶质纤维酸性蛋白（GFAP）和谷氨酰胺合成酶（GS）的发育表达。结果发现，这两种星形胶质细胞标志物在体外不同发育阶段共表达。在细胞成熟阶段（即第14天至34天之间），GFAP水平和GS活性迅速且平行增加。同时，免疫反应性细胞数量增加，而早期培养物中染色的长而粗的突起逐渐消失。目前的结果表明，在这个特定的细胞培养系统中，只发育出一种同时表达GFAP和GS且达到相对高度成熟的星形胶质细胞。
