Olğaç Kemal Tuna, Akçay Ergun, Çil Beste, Uçar Burak Mehmet, Daşkın Ali
Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Ankara University, 06110 Dışkapı/Ankara, Turkey.
Diyarbakır Hippodrome, Race Horse Hospital, Economic Establishment of Horse Breeding and Racig of Turkey Jockey Club Association, 21750 Çınar/Diyarbakır, Turkey.
J Anim Sci Technol. 2017 Oct 9;59:21. doi: 10.1186/s40781-017-0146-4. eCollection 2017.
The aim of the study is to evaluate the effectiveness of thermographic monitoring, using the temperature changes of perianal and perivulvar areas for the determination of estrus in Anatolian Shepherd bitches. Fifteen bitches were used in the study. Blood and vaginal smear samples were collected and thermographic monitoring of perianal and perivulvar areas were carried out starting from proestrus to early diestrus. Also, external signs of estrus were investigated. Smear samples were evaluated by light microscopy after Diff-Quik staining method and superficial and keratinized superficial cells were determined as percentage (S + KS%). Progesterone and luteinizing hormone measurements were done by radioimmunoassay. The difference in temperature between perianal and perivulvar areas was evaluated through thermographic images by FLIR ResearchIR Software.
According to the results obtained from the study, differences between progesterone and S + KS% were statistically significant ( < 0,05). Although temperature showed increase and decrease with progesterone and S + KS%, the differences were not important statistically ( > 0,05). Serum luteinizing hormone levels did not sign any difference ( > 0,05).
As a result, thermographic monitoring alone is not enough for estrus detection in Anatolian Shepherd bitches. However, it can be used to assist the actual estrus detection technique in terms of providing some foreknowledge by evaluating the differences in temperature.
本研究的目的是评估热成像监测的有效性,利用肛周和阴门周围区域的温度变化来确定安纳托利亚牧羊犬母犬的发情期。本研究使用了15只母犬。采集血液和阴道涂片样本,从发情前期到发情后期对肛周和阴门周围区域进行热成像监测。此外,还对发情的外部迹象进行了调查。涂片样本在采用Diff-Quik染色法后通过光学显微镜进行评估,并将表层和角质化表层细胞确定为百分比(S + KS%)。通过放射免疫测定法进行孕酮和促黄体生成素的测量。通过FLIR ResearchIR软件利用热成像图像评估肛周和阴门周围区域之间的温度差异。
根据本研究获得的结果,孕酮和S + KS%之间的差异具有统计学意义(<0.05)。尽管温度随孕酮和S + KS%出现升高和降低,但差异在统计学上并不显著(>0.05)。血清促黄体生成素水平未显示任何差异(>0.05)。
因此,单独的热成像监测不足以用于安纳托利亚牧羊犬母犬的发情检测。然而,通过评估温度差异提供一些预知信息,它可用于辅助实际的发情检测技术。