通过下一代测序技术绘制DNA断裂图谱。
Mapping DNA Breaks by Next-Generation Sequencing.
作者信息
Baranello Laura, Kouzine Fedor, Wojtowicz Damian, Cui Kairong, Zhao Keji, Przytycka Teresa M, Capranico Giovanni, Levens David
机构信息
Laboratory of Pathology, NCI/NIH, Bethesda, MD, 20892, USA.
Department of Cellular and Molecular Biology, Karolinska Institutet, 17177, Stockholm, Sweden.
出版信息
Methods Mol Biol. 2018;1672:155-166. doi: 10.1007/978-1-4939-7306-4_13.
Abstract
Here, we present two approaches to map DNA double-strand breaks (DSBs) and single-strand breaks (SSBs) in the genome of human cells. We named these methods respectively DSB-Seq and SSB-Seq. We tested the DSB and SSB-Seq in HCT1116, human colon cancer cells, and validated the results using the topoisomerase 2 (Top2)-poisoning agent etoposide (ETO). These methods are powerful tools for the direct detection of the physiological and pathological "breakome" of the DNA in human cells.
摘要
在此，我们展示了两种在人类细胞基因组中绘制DNA双链断裂（DSB）和单链断裂（SSB）图谱的方法。我们分别将这些方法命名为DSB-Seq和SSB-Seq。我们在人结肠癌细胞HCT1116中测试了DSB和SSB-Seq，并使用拓扑异构酶2（Top2）中毒剂依托泊苷（ETO）验证了结果。这些方法是直接检测人类细胞中DNA生理和病理“断裂组”的强大工具。
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