通过水解末端测序法对掺入DNA中的核糖核苷酸进行定位
Mapping Ribonucleotides Incorporated into DNA by Hydrolytic End-Sequencing.
作者信息
Orebaugh Clinton D, Lujan Scott A, Burkholder Adam B, Clausen Anders R, Kunkel Thomas A
机构信息
Genome Integrity and Structural Biology Laboratory, National Institute for Environmental Health Sciences, National Institute of Health (NIH), 111 TW Alexander Drive, Research Triangle Park, NC, 27709, USA.
Integrative Bioinformatics, National Institute for Environmental Health Sciences, National Institute of Health (NIH), Research Triangle Park, NC, USA.
出版信息
Methods Mol Biol. 2018;1672:329-345. doi: 10.1007/978-1-4939-7306-4_23.
Ribonucleotides embedded within DNA render the DNA sensitive to the formation of single-stranded breaks under alkali conditions. Here, we describe a next-generation sequencing method called hydrolytic end sequencing (HydEn-seq) to map ribonucleotides inserted into the genome of Saccharomyce cerevisiae strains deficient in ribonucleotide excision repair. We use this method to map several genomic features in wild-type and replicase variant yeast strains.
嵌入DNA中的核糖核苷酸使DNA在碱性条件下对单链断裂的形成敏感。在这里，我们描述了一种名为水解末端测序（HydEn-seq）的新一代测序方法，用于绘制插入缺乏核糖核苷酸切除修复的酿酒酵母菌株基因组中的核糖核苷酸图谱。我们使用这种方法来绘制野生型和复制酶变体酵母菌株中的几个基因组特征。
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