类风湿关节炎患者的II类人类白细胞抗原基因和T细胞受体多态性

Class II human leukocyte antigen genes and T cell receptor polymorphisms in patients with rheumatoid arthritis.

作者信息

Gao X J, Ball E J, Dombrausky L, Olsen N J, Pincus T, Khan M A, Wolfe F, Stastny P

机构信息

Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas 75235.

出版信息

Am J Med. 1988 Dec 23;85(6A):14-6. doi: 10.1016/0002-9343(88)90373-7.

DOI:10.1016/0002-9343(88)90373-7

PMID:2904760

Abstract

Human leukocyte antigen (HLA) typing was performed in 174 patients with rheumatoid arthritis and 222 white control subjects. Increases in HLA-DR4 and HLA-DR1 were observed as in previous studies. Each of these appeared to be inherited as dominant risk factors. Southern blotting with a DR-beta probe after digestion of genomic DNA with the restriction enzyme Bam HI showed seven bands. Three of them correlated with DR4 and were increased in rheumatoid arthritis patients. Subsets of DR4 were determined by polymerase chain reaction amplification followed by hybridization with oligonucleotide probes. Dw4 was increased in rheumatoid arthritis patients, and the frequency of the other subsets appeared to be similar in rheumatoid arthritis patients and control subjects. A polymorphism associated with the T cell receptor V-beta-8 gene family was significantly increased in rheumatoid arthritis patients.

摘要

对174例类风湿关节炎患者和222名白人对照者进行了人类白细胞抗原（HLA）分型。如先前研究一样，观察到HLA - DR4和HLA - DR1有所增加。它们似乎均作为显性风险因素遗传。用限制性内切酶Bam HI消化基因组DNA后，用DR - β探针进行Southern印迹分析显示出七条带。其中三条与DR4相关，且在类风湿关节炎患者中增加。通过聚合酶链反应扩增，然后与寡核苷酸探针杂交来确定DR4的亚型。类风湿关节炎患者中Dw4增加，而其他亚型在类风湿关节炎患者和对照者中的频率似乎相似。与T细胞受体V - β - 8基因家族相关的一种多态性在类风湿关节炎患者中显著增加。