来自神经胶质细胞、神经元胞体细胞和突触体的γ-氨基丁酸转氨酶的比较研究。

Comparative study of GABA-T from glial cells, neuronal perikarya cells and synaptosomes.

Bedoya M P, Toledo C, de Vicente M, González M P

Facultad de Farmacia, Instituto de Bioquímica (Centro mixto C.S.I.C.-U.C.M.), Madrid, Spain.

Comp Biochem Physiol C Comp Pharmacol Toxicol. 1988;90(1):61-4. doi: 10.1016/0742-8413(88)90097-7.

Abstract

This paper presents a fast method of brain cell separation and a comparative study of GABA-T from different cellular compartments (glial cells, neuronal perikarya cells and synaptosomes). 2. The method of cellular separation offers the advantages of rapidity, ease and reproducibility. 3. The GABA-T from the three studied compartments had similar kinetic characteristics in respect of their Kms and Vmaxs. 4. The GABA-T needs PLP to reach its maximum activity; this dependence is related to the enzyme localization.

摘要

本文介绍了一种快速分离脑细胞的方法，并对来自不同细胞区室（神经胶质细胞、神经元胞体细胞和突触体）的GABA-T进行了比较研究。2. 细胞分离方法具有快速、简便和可重复性的优点。3. 所研究的三个区室中的GABA-T在Km和Vmax方面具有相似的动力学特征。4. GABA-T需要磷酸吡哆醛才能达到其最大活性；这种依赖性与酶的定位有关。