Xiao Yan, Ding Liang, Chen Hao, Gu Yi-Huang
The Second Clinical Medical School, Nanjing University of Chinese Medicine, Nanjing 210023, China.
Literature Research Institute, Nanjing University of Chinese Medicine, Nanjing 210023.
Zhen Ci Yan Jiu. 2016 Dec 25;41(6):502-8.
To observe the effectiveness of grain-moxibustion in resisting oxidative stress in doxorubicin (DOX)-induced cardiomyopathy rats.
Sixty Sprague-Dawley (SD) rats were randomly divided into blank control group (blank group), cardiomyopathy model (model) group and grain-moxibustion (moxibustion) group, with 20 rats in each one. The cardiomyopathy model was established by intraperitoneal injection of DOX (0.5 mg/100 g), once a week for 4 weeks. Grain-moxibustion was applied to "Guanyuan" (CV 4) and "Juque" (CV 14) for 5 cones, once daily for 4 weeks except the Sundays. The rats' heart rate (HR), left ventricular systolic pressure (LVSP), left ventricular diastolic pressure (LVDP), left ventricular pressure maximum rising rate (+dp/dt), left ventricular pressure maximum decline rate (-dp/dt), left ventricular pressure change time (t-dp/dt) were detected to evaluate the cardiac function by using a physiological signal acquisition-analysis system. The pathological changes of the myocardium were observed by optical microscopy after H.E. staining. The activity of serum lactate dehydrogenase(LDH), creatine kinase(CK) and myocardial superoxide dismutase (SOD), and myocardial malondialdehyde (MDA) and serum cardiac troponin Ⅰ(cTnⅠ) contents were assayed by enzyme-linked immuno sorbent assay kit (ELISA).
Following modeling, the HR, LVSP, +dp/dt, -dp/dt, and myocardial SOD activity were significantly down-regulated (<0.05, <0.01), while the LVDP, t-dp/dt, serum LDH and CK activity and cTnⅠ content, as well as myocardial MDA content were considerably increased in comparison with the blank group (<0.05, <0.01). After moxibustion intervention, the decreased HR, LVSP, +dp/dt, -dp/dt, and myocardial SOD activity levels and the increased LVDP, t-dp/dt, serum LDH and CK activity and cTnⅠ content, as well as myocardial MDA content were remarkably suppressed in comparison with the model group (<0.05, <0.01).
Grain-moxibustion can significantly improve the left ventricular function, increase heart rate in cardiomyopathy rats, which may be associated with its effects in reducing myocardial oxidative stress damage by down-regulating the content of MDA and up-regulating SOD activity in the heart.
观察麦粒灸对阿霉素（DOX）诱导的心肌病大鼠抗氧化应激的作用。
将60只Sprague-Dawley（SD）大鼠随机分为空白对照组（空白组）、心肌病模型组（模型组）和麦粒灸组（艾灸组），每组20只。采用腹腔注射DOX（0.5 mg/100 g）建立心肌病模型，每周1次，共4周。麦粒灸于“关元”（CV 4）和“巨阙”（CV 14）施灸5壮，除周日外每日1次，共4周。使用生理信号采集分析系统检测大鼠心率（HR）、左心室收缩压（LVSP）、左心室舒张压（LVDP）、左心室压力最大上升速率（+dp/dt）、左心室压力最大下降速率（-dp/dt）、左心室压力变化时间（t-dp/dt）以评估心功能。苏木精-伊红（H.E.）染色后光学显微镜观察心肌病理变化。采用酶联免疫吸附测定试剂盒（ELISA）检测血清乳酸脱氢酶（LDH）、肌酸激酶（CK）活性及心肌超氧化物歧化酶（SOD）活性，以及心肌丙二醛（MDA）含量和血清心肌肌钙蛋白Ⅰ（cTnⅠ）含量。
造模后，与空白组相比，HR、LVSP、+dp/dt、-dp/dt及心肌SOD活性显著下调（<0.05，<0.01），而LVDP、t-dp/dt、血清LDH和CK活性及cTnⅠ含量以及心肌MDA含量显著升高（<0.05，<0.01）。艾灸干预后，与模型组相比，HR、LVSP、+dp/dt、-dp/dt及心肌SOD活性水平的降低以及LVDP、t-dp/dt、血清LDH和CK活性及cTnⅠ含量以及心肌MDA含量的升高均受到显著抑制（<0.05，<0.01）。
麦粒灸可显著改善心肌病大鼠左心室功能，提高心率，这可能与其下调心脏MDA含量、上调SOD活性从而减轻心肌氧化应激损伤有关。
