Laukamm-Josten U, Bain O
Landesinstitut für Tropenmedizin, Berlin, FRG.
Acta Trop. 1988 Dec;45(4):373-7.
The murid model of Monanema martini in Lemniscomys striatus was used to evaluate its potential as drug screening model in onchocerciasis. It had been described that the histopathology and the reaction to diethylcarbamazine treatment of this model closely resemble human onchocerciasis. To study further similarities the in vitro effect of midazolam was examined. Skin-dwelling microfilariae (mf) of M. martini were taken by skin snips and placed in either plain phosphat buffered saline or midazolam. Concentrations of 50 micrograms/ml midazolam significantly reduced motility within 15 min. The percentage of fully motile mf dropped to 9.2 and 1.4 after 15 and 30 min, respectively. In contrast to this finding fully motile mf were obtained after intraperitoneal injection of 60 mg/kg BW; but the technique used did not allow to evaluate the in vivo effect of midazolam. The similarities with the human disease and the finding that midazolam paralyses mf of M. martini like mf of O. volvulus in vitro indicate the potential of the model for simulating human onchocerciasis.
采用条纹林鼠作为马氏盘尾丝虫病的啮齿动物模型，以评估其作为盘尾丝虫病药物筛选模型的潜力。已有描述称，该模型的组织病理学以及对乙胺嗪治疗的反应与人类盘尾丝虫病极为相似。为进一步研究两者的相似性，对咪达唑仑的体外效应进行了检测。通过皮肤剪取获取马氏盘尾丝虫的皮肤微丝蚴，并将其置于普通磷酸盐缓冲盐溶液或咪达唑仑中。50微克/毫升的咪达唑仑浓度在15分钟内显著降低了微丝蚴的活力。15分钟和30分钟后，完全活动的微丝蚴百分比分别降至9.2%和1.4%。与这一结果相反，腹腔注射60毫克/千克体重后可获得完全活动的微丝蚴；但所采用的技术无法评估咪达唑仑的体内效应。该模型与人类疾病的相似性以及咪达唑仑在体外可使马氏盘尾丝虫的微丝蚴像旋盘尾丝虫的微丝蚴一样麻痹，这表明该模型具有模拟人类盘尾丝虫病的潜力。
