开发一种基于多重肿瘤相关自身抗体的血液检测方法，用于结直肠癌的检测。
Development of a multiplexed tumor-associated autoantibody-based blood test for the detection of colorectal cancer.
机构信息
Division of Colorectal Surgery, Chang Gung Memorial Hospital, Keelung and Chang Gung University, College of Medicine, Taoyuan, Taiwan.
Genpronex Biomedical Inc, Taiwan.
出版信息
Clin Chim Acta. 2017 Dec;475:157-163. doi: 10.1016/j.cca.2017.10.022. Epub 2017 Oct 23.
BACKGROUND
Colorectal cancer (CRC) is one of the most common malignancies worldwide, and early diagnosis is vital to improving prognoses. We explored the diagnostic potential of a multiplex autoantibody panel as a biomarker for the detection of CRC by ELISA.
METHODS
In total, 192 serum samples (92 CRC and 100 matched controls) were tested against a panel of 12 tumor-associated antigens (TAAs): RPH3AL, RPL36, SLP2, p53, survivin, ANAXA4, SEC61B, CCCAP, NYCO16, NMDAR, PLSCR1, and HDAC5. Individual and combined autoantibody signatures were examined.
RESULTS
Compared to individual autoantibody markers, the combinations of TAAs provided better discrimination between tumorous and normal sera. The overall sensitivity of a selected panel of four antibodies (anti-SLP2, -p53, -SEC61B, and -PLSCR1) was 64.1%, with a specificity of 80% that increased to 83.7% when carcinoembryonic antigen (CEA) measurement was added. Furthermore, the sensitivity of the panel of four antibodies for early and advanced stages of CRC was 66.7% and 62%, increasing to 88.3% and 84%, respectively, when CEA was added.
CONCLUSIONS
We identified a panel of four antibodies as a promising diagnostic biomarker for the detection of CRC.
背景
结直肠癌（CRC）是全球最常见的恶性肿瘤之一，早期诊断对于改善预后至关重要。我们通过 ELISA 探索了多重自身抗体谱作为 CRC 检测生物标志物的诊断潜力。
方法
共检测了 192 份血清样本（92 份 CRC 和 100 份匹配的对照），这些样本针对 12 种肿瘤相关抗原（TAAs）的抗体面板进行了检测：RPH3AL、RPL36、SLP2、p53、survivin、ANAXA4、SEC61B、CCCAP、NYCO16、NMDAR、PLSCR1 和 HDAC5。检测了单个和组合的自身抗体特征。
结果
与单个自身抗体标志物相比，TAAs 的组合提供了更好的肿瘤与正常血清之间的区分。选定的四个抗体（抗 SLP2、-p53、-SEC61B 和 -PLSCR1）组合的总体敏感性为 64.1%，特异性为 80%，当加入癌胚抗原（CEA）测量时，特异性增加至 83.7%。此外，该 panel 的四个抗体对 CRC 的早期和晚期阶段的敏感性分别为 66.7%和 62%，当加入 CEA 时，敏感性分别增加至 88.3%和 84%。
结论
我们确定了一个由四个抗体组成的 panel 作为检测 CRC 的有前途的诊断生物标志物。
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