School of Agriculture, Yunnan University, Kunming, 650091, P. R. China.
School of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong, 723001, P. R. China.
J Microbiol. 2017 Nov;55(11):871-876. doi: 10.1007/s12275-017-7173-1. Epub 2017 Oct 27.
In the present study, we identified genes that are putatively involved in the production of fungal 10-hydroxycamptothecin via transcriptome sequencing and characterization of the Xylaria sp. M71 treated with salicylic acid (SA). A total of 60,664,200 raw reads were assembled into 26,044 unigenes. BLAST assigned 8,767 (33.7%) and 10,840 (41.6%) unigenes to 40 Gene Ontology (GO) annotations and 108 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, respectively. A total of 3,713 unigenes comprising 1,504 upregulated and 2,209 downregulated unigenes were found to be differentially expressed between SA-induced and control fungi. Based on the camptothecin biosynthesis pathway in plants, 13 functional genes of Xylaria sp. M71 were mapped to the mevalonate (MVA) pathway, suggesting that the fungal 10-hydroxycamptothecin is produced via the MVA pathway. In summary, analysis of the Xylaria sp. M71 transcriptome allowed the identification of unigenes that are putatively involved in 10-hydroxycamptothecin biosynthesis in fungi.
在本研究中，我们通过对水杨酸（SA）处理的黄韧革菌 M71 的转录组测序和表征，鉴定了可能参与真菌 10-羟基喜树碱产生的基因。总共获得了 60664200 条原始reads，组装成 26044 条 unigenes。BLAST 将 8767（33.7%）和 10840（41.6%）条 unigenes分别分配到 40 个基因本体（GO）注释和 108 个京都基因与基因组百科全书（KEGG）途径中。在 SA 诱导和对照真菌之间，共发现 3713 条 unigenes，包括 1504 条上调和 2209 条下调 unigenes，这些 unigenes表现出差异表达。基于植物中的喜树碱生物合成途径，将黄韧革菌 M71 的 13 个功能基因映射到甲羟戊酸（MVA）途径，表明真菌 10-羟基喜树碱是通过 MVA 途径产生的。总之，对黄韧革菌 M71 转录组的分析鉴定了可能参与真菌 10-羟基喜树碱生物合成的 unigenes。
