电化学检测血浆免疫球蛋白作为阿尔茨海默病的生物标志物。
Electrochemical Detection of Plasma Immunoglobulin as a Biomarker for Alzheimer's Disease.
机构信息
School of Chemical Engineering and Advance Materials, Newcastle University, Newcastle upon Tyne NE1 7RU, UK.
Department of Chemical and Process Engineering, Faculty of Engineering, Bilecik Şeyh Edebali University, 11230 Bilecik, Turkey.
出版信息
Sensors (Basel). 2017 Oct 27;17(11):2464. doi: 10.3390/s17112464.
The clinical diagnosis and treatment of Alzheimer's disease (AD) represent a challenge to clinicians due to the variability of clinical symptomatology as well as the unavailability of reliable diagnostic tests. In this study, the development of a novel electrochemical assay and its potential to detect peripheral blood biomarkers to diagnose AD using plasma immunoglobulins is investigated. The immunosensor employs a gold electrode as the immobilizing substrate, albumin depleted plasma immunoglobulin as the biomarker, and polyclonal rabbit Anti-human immunoglobulin (against IgA, IgG, IgM) as the receptor for plasma conjugation. The assay showed good response, sensitivity and reproducibility in differentiating plasma immunoglobulin from AD and control subjects down to 10 dilutions of plasma immunoglobulin representing plasma content concentrations in the pg mL range. The newly developed assay is highly sensitive, less time consuming, easy to handle, can be easily modified to detect other dementia-related biomarkers in blood samples, and can be easily integrated into portable devices.
阿尔茨海默病（AD）的临床诊断和治疗对临床医生来说是一个挑战，这是由于临床症状的可变性以及缺乏可靠的诊断测试。在这项研究中，开发了一种新的电化学分析方法，该方法可能通过检测血浆免疫球蛋白来检测外周血生物标志物，从而诊断 AD。该免疫传感器采用金电极作为固定化基底，白蛋白耗尽的血浆免疫球蛋白作为生物标志物，多克隆兔抗人免疫球蛋白（针对 IgA、IgG、IgM）作为血浆结合的受体。该测定法在区分 AD 和对照受试者的血浆免疫球蛋白方面表现出良好的响应、灵敏度和重现性，可将血浆免疫球蛋白的稀释度低至 10 倍，代表 pg mL 范围内的血浆含量浓度。新开发的测定法具有高灵敏度、耗时少、易于操作、可轻松修改以检测血液样本中的其他与痴呆相关的生物标志物，并且易于集成到便携式设备中。
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