Ebisawa I, Takayanagi M, Kigawa M
Department of Public Health, Toho University School of Medicine, Tokyo, Japan.
Jpn J Exp Med. 1988 Dec;58(6):233-41.
Clostridium tetani was isolated from human and animal stools at the following rates [95% confidence interval (CI)]: Human, 0% (1.5-0); horse, 1% (5-0); cow in cowshed, 4% (10-1); cow in pasture, 8.3% (17-1), calf in pasture, 0% (7-0); dog, 2% (11-0) and sheep in pasture, 25% (44-14). Quantification of C. tetani in 16 animal stools positive for the bacillus was impossible in most cases, as the number of tetanus bacilli present was not large enough for this purpose. Contaminating anaerobic and facultative anaerobic bacteria in human and animal stools, i.e., C. perfringens and Streptococcus sp., Group G, inhibited isolation of C. tetani from these materials, particularly at the step of isolation employing its swarming character.
破伤风梭菌从人和动物粪便中分离出来的比例如下[95%置信区间（CI）]：人，0%（1.5 - 0）；马，1%（5 - 0）；牛棚中的牛，4%（10 - 1）；牧场中的牛，8.3%（17 - 1），牧场中的小牛，0%（7 - 0）；狗，2%（11 - 0）；牧场中的羊，25%（44 - 14）。在大多数情况下，无法对16份检测出该杆菌呈阳性的动物粪便中的破伤风梭菌进行定量，因为存在的破伤风杆菌数量不足以进行此项操作。人和动物粪便中的污染性厌氧菌和兼性厌氧菌，即产气荚膜梭菌和G组链球菌，会抑制从这些材料中分离出破伤风梭菌，尤其是在利用其蔓延特性进行分离的步骤中。
