Construction of FWPV Chimaeric MVA.
作者信息
Buttigieg Karen R, Skinner Michael A
机构信息
Section of Virology, Faculty of Medicine, Imperial College London, London, UK.
出版信息
Bio Protoc. 2015 Jan 5;5(1). doi: 10.21769/BioProtoc.1376.
Abstract
Construction of chimaeric MVA is a useful tool with which to study gene function of related viruses. The protocol given here describes MVA chimaeras containing genes from Fowlpox virus (FWPV), although this can be applied to DNA derived from other organisms. There are a number of steps required to make the chimaeric MVA: 1) Purification of viral particles; 2) Extraction of DNA from purified viral particles; 3) Assembly of linear recombination templates; 4) Transfection of linear recombination templates; 5) Selection of chimaeric MVA. .
摘要
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本文引用的文献
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