Craig J, Fowler S, Skinner J D, Burgoyne L A, McInnes J L
School of Biological Sciences, Flinders University of South Australia, Bedford Park.
Appl Theor Electrophor. 1988;1(1):23-8.
DNA probes to multiple hypervariable human genomic loci are potentially highly informative as regards individual genome identification and parentage studies. However the resultant Southern blot patterns are generally highly complex and their interpretation difficult, with one important limitation being the extent of fragment separation and resolution. The separation of large DNA restriction fragments in agarose gels may be improved, in comparison to separations obtained by conventional electrophoresis, by the use of field inversion techniques. This is demonstrated by the analysis of the complex human Satellite III related DNA polymorphism. Detection of the Satellite III related restriction fragments is achieved either by using a [35S]-labelled probe (228S) or by using the same probe in a convenient non-isotopic form constructed by the photobiotin process. In addition, the probe 228S is useful for sexing the human genome, by the identification of a Y-specific restriction fragment.
针对多个高变人类基因组位点的DNA探针在个体基因组鉴定和亲子鉴定研究方面可能具有高度的信息价值。然而，由此产生的Southern印迹图谱通常非常复杂，难以解读，一个重要的限制因素是片段分离和分辨率的程度。与传统电泳获得的分离效果相比，通过使用场反转技术可以改善琼脂糖凝胶中大型DNA限制性片段的分离。对复杂的人类卫星III相关DNA多态性的分析证明了这一点。通过使用[35S]标记的探针（228S）或通过使用通过光生物素方法构建的方便的非同位素形式的相同探针，可以实现对卫星III相关限制性片段的检测。此外，探针228S可通过鉴定Y特异性限制性片段来用于人类基因组的性别鉴定。
