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在伊朗西北部阿尔达比勒地区首次在 spp 中检测到 。 (你提供的原文中部分内容缺失,我只能按现有内容翻译)

First detection of in spp in Ardabil area, northwest of Iran.

作者信息

Gholamreza Shahbazi, Somaieh Matin, Roya Shahbazi, Alireza Babapour, Ghazale Adhami, Yasin Bageri

机构信息

Department of Parasitology and Mycology, Faculty of Veterinary Medicine, Islamic Azad University, Science and Research Branch, Tehran, Iran.

Department of Internal Medicine, Ardabil University of Medical Sciences, Ardabil, Iran.

出版信息

J Vector Borne Dis. 2017 Jul-Sep;54(3):277-281. doi: 10.4103/0972-9062.217620.

Abstract

BACKGROUND & OBJECTIVES: Babesia is an intraerythrocytic protozoan parasite which is transmitted by hard ticks of the Ixodidae family. One of the problems associated with protozoan infection is the determination and characterization of the vectors. The aim of the present study was to detect Babesia ovis in the salivary gland of Dermacentor spp.

METHODS

A total of 200 adult Dermacentor ticks (139 D. niveus and 61 D. marginatus) were collected from sheep suspected to be infected with babesiosis in the Ardabil region of Iran from April to September 2015 (active season of ticks); and were identified using standard taxonomic keys. Deoxyribonucleic acid (DNA) was isolated from the salivary glands of ticks and analyzed with the primers derived from the hyper variable V4 region of 18S ribosomal ribonucleic acid (18S rRNA) of the Babesia species using polymerase chain reaction (PCR).

RESULTS

Babesia ovis was detected in 5.8% of the D. niveus and 3.3% of the D. marginatus positive samples in the second round of semi-nested PCR.

INTERPRETATION & CONCLUSION: Based on the results obtained from this study, it is concluded that D. niveus and D. marginatus, which are distributed in Ardabil region of Iran, might play a major role in the transmission of infection as a natural vector of B. ovis.

摘要

背景与目的

巴贝斯虫是一种红细胞内寄生的原生动物寄生虫,通过硬蜱科的硬蜱传播。原生动物感染相关的问题之一是病媒的确定和特征描述。本研究的目的是检测 Dermacentor 属蜱的唾液腺中的绵羊巴贝斯虫。

方法

2015 年 4 月至 9 月(蜱的活跃季节),从伊朗阿尔达比勒地区疑似感染巴贝斯虫病的绵羊身上采集了总共 200 只成年 Dermacentor 蜱(139 只银盾革蜱和 61 只边缘革蜱);并使用标准分类学鉴定方法进行鉴定。从蜱的唾液腺中分离脱氧核糖核酸(DNA),并使用聚合酶链反应(PCR),用源自巴贝斯虫物种 18S 核糖体核糖核酸(18S rRNA)高变 V4 区域的引物进行分析。

结果

在第二轮半巢式 PCR 中,在 5.8%的银盾革蜱和 3.3%的边缘革蜱阳性样本中检测到绵羊巴贝斯虫。

解读与结论

根据本研究获得的结果,得出结论:分布在伊朗阿尔达比勒地区的银盾革蜱和边缘革蜱可能作为绵羊巴贝斯虫的自然传播媒介在感染传播中起主要作用。

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