Surveillance of variant mecA gene and analysis of efflux pump in mecA negative MRSA infections.

It has been known from the medical records of Kancheepuram district hospitals that there is a prevalence of Methicillin resistant Staphylococcus aureus strain. Now the recent epidemiology has shown the presence of Methicillin Resistant Staphylococcus aureus even in school going children community individuals. In view of the above situation, Methicillin resistant Staphylococcus aureus screening was undertaken in the above said region. Both samples from hospital sources and school going children were screened for Methicillin Resistant Staphylococcus aureus. The results have shown that the samples had multiantibiotic resistant property along with methicillin resistance. The genomic studies have revealed that there were of positive genomic sequence for mec A and 5 isolates for mecA negative gene sequence. This would assume that even negative mec A genome has the resistant expression. Out of the 5 isolates two isolates have shown the presence of both nor A and qac A/B genes. The above results are discussed with efflux of drugs and future proposal on proteomics.