Wan Yong, Rogers Matthew B, Szabo-Rogers Heather L
Center for Craniofacial Regeneration, Department of Oral Biology, School of Dental Medicine, University of Pittsburgh, Pittsburgh, PA, 15213, United States.
Center for Microbiome, Department of Surgery, School of Medicine, University of Pittsburgh, Pittsburgh, PA, 15213, United States.
Gene Expr Patterns. 2018 Jan;27:46-55. doi: 10.1016/j.gep.2017.10.004. Epub 2017 Nov 6.
The nose is the central feature of the amniote face. In adults, the nose is a structurally and functionally complex organ that consists of bone, cartilage, glands and ducts. In an ongoing expression screen in our lab, we found several novel markers for specific tissues in the nasal region. Here, using in situ hybridization expression experiments, we report that Alx1, Ap-2β, Crispld1, Eya4, Moxd1, and Penk have tissue specific expression during murine nasal development. At E11.5, we observed that Alx1, Ap-2β, Crispld1, and Eya4 are expressed in the medial and lateral nasal prominences. We found that Moxd1 and Penk are expressed in the lateral nasal prominences. At E15.5, Alx1 is expressed in nasal septum. Ap-2β and Crispld1 are expressed in nasal glands and cartilages. Eya4 is expressed in olfactory epithelium. Intriguingly at E15.5 Moxd1 is expressed in all the nasal cartilage while the expression of Penk is restricted to chondrocytes contributing to the posterior nasal septum. The expression domains reported here suggest that these genes warrant functional studies to determine their role in nasal capsule morphogenesis.
鼻子是羊膜动物面部的主要特征。在成体中，鼻子是一个结构和功能复杂的器官，由骨骼、软骨、腺体和导管组成。在我们实验室正在进行的表达筛选中，我们发现了鼻腔区域特定组织的几个新标记物。在此，我们通过原位杂交表达实验报告，Alx1、Ap - 2β、Crispld1、Eya4、Moxd1和Penk在小鼠鼻腔发育过程中具有组织特异性表达。在胚胎第11.5天（E11.5），我们观察到Alx1、Ap - 2β、Crispld1和Eya4在鼻内侧和外侧隆起中表达。我们发现Moxd1和Penk在鼻外侧隆起中表达。在胚胎第15.5天（E15.5），Alx1在鼻中隔中表达。Ap - 2β和Crispld1在鼻腺和软骨中表达。Eya4在嗅觉上皮中表达。有趣的是，在E15.5时，Moxd1在所有鼻软骨中表达，而Penk的表达仅限于对后鼻中隔有贡献的软骨细胞。这里报道的表达域表明，这些基因值得进行功能研究，以确定它们在鼻囊形态发生中的作用。
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