Nichols India S, Jones Mary I, Okere Chuma, Ananaba Godwin, Bush Brittany, Gray Cloe, Brager Allison, Ehlen J Christopher, Paul Ketema
Department of Biological Sciences, Clark Atlanta University, Atlanta, Georgia, United States of America.
Department of Neurobiology, Morehouse School of Medicine, Atlanta, Georgia, United States of America.
PLoS One. 2017 Nov 10;12(11):e0187071. doi: 10.1371/journal.pone.0187071. eCollection 2017.
Nitrergic neurons of the dorsal raphe nucleus (DRN) may play a role in physiological stress responses. The caudal lateral wings (CLW) are unique compared to other rostral-caudal DRN sub-regions because they contain distinct nitric oxide (NO) synthase (NOS) populations that are independent of tryptophan hydroxylase (TPH). NOS neurons in the CLW are also highly activated during acute restraint stress. However, the effects of acute stress duration on NOS activation in the CLW are unclear. Here NADPH-d, an index of NOS activity, is used to show that sub-regions of the DRN have differential NOS activation in response to 6 hours of restraint stress in rats. We report increased NOS activity through 6 hours of restraint in the caudal lateral wings and ventromedial sub-regions. These data suggest that, NOS neurons may play a dynamic role in the response to stress duration.
中缝背核（DRN）的一氧化氮能神经元可能在生理应激反应中发挥作用。与中缝背核其他头-尾亚区域相比，尾外侧翼（CLW）具有独特性，因为它们含有独立于色氨酸羟化酶（TPH）的不同一氧化氮（NO）合酶（NOS）群体。CLW中的NOS神经元在急性束缚应激期间也会高度激活。然而，急性应激持续时间对CLW中NOS激活的影响尚不清楚。在这里，使用NADPH-d（一种NOS活性指标）来表明，中缝背核的亚区域在大鼠6小时的束缚应激反应中具有不同的NOS激活情况。我们报告，在尾外侧翼和腹内侧亚区域，经过6小时的束缚，NOS活性增加。这些数据表明，NOS神经元可能在对应激持续时间的反应中发挥动态作用。
