Paranzini Cristiane Sella, Correia Luiz Eduardo Cruz Dos Santos, de Camargo Laiza Sartori, Silva Kelry Mayara, de França Tatyana Mendes, Silva Josineudson Augusto de Vasconcelos, Veiga Nabor, de Souza Fabiana Ferreira
Department of Animal Reproduction and Veterinary Radiology, FMVZ, São Paulo State University, UNESP, Botucatu, Brazil.
Department of Animal Science, FCAV, São Paulo State University, UNESP, Jaboticabal, Brazil.
Theriogenology. 2018 Feb;107:36-40. doi: 10.1016/j.theriogenology.2017.10.032. Epub 2017 Oct 28.
This study aimed to report in detail, the technique and challenges of cloacal massage, to collect and evaluate semen from red-winged tinamou (Rhynchotus rufescens) keep in captivity, performed by only one technician. Sixty-four semen collection attempts, from 16 adult males, during breeding season and 16 attempts form these same 16 males in non-breeding season, were performed. Prior to collection, all animals were conditioned to cloacal massage for 6 weeks and the ejaculates were succeed with viable spermatozoa and then, evaluated for feces, urine and mucus contamination, volume, concentration, sperm vigor, motility, morphological defects and acrosome integrity. Semen collection success rate was 63% in breeding season and 2 (5%) samples were discarded by grade 5 contamination. Only 3 ejaculates from 16 tinamou were obtained in non-breeding season. Sperm concentration and acrosome integrity was higher (p = 0.00) in breeding season, and the percentage of total sperm morphological defects, were high in both in breeding and out breeding season. Overall, we concluded that the red-winged tinamou breeding season, is linked to photoperiod (spring and summer), and at this period time, semen can be obtained by cloacal massage collection satisfactorily, allowing its use in reproduction biotechnologies and sperm cryopreservation.
本研究旨在详细报告泄殖腔按摩的技术和挑战，由一名技术人员对圈养的红翅䳍（Rhynchotus rufescens）进行精液采集和评估。在繁殖季节对16只成年雄性红翅䳍进行了64次精液采集尝试，在非繁殖季节对这16只相同的雄性红翅䳍进行了16次采集尝试。在采集之前，所有动物都接受了6周的泄殖腔按摩训练，采集到的精液含有活精子，随后对精液进行粪便、尿液和黏液污染、体积、浓度、精子活力、运动能力、形态缺陷和顶体完整性评估。繁殖季节精液采集成功率为63%，2份（5%）样本因5级污染而被丢弃。在非繁殖季节，16只䳍中仅获得3份射精样本。繁殖季节精子浓度和顶体完整性较高（p = 0.00），繁殖季节和非繁殖季节精子形态缺陷总数的百分比均较高。总体而言，我们得出结论，红翅䳍的繁殖季节与光周期（春季和夏季）有关，在此期间，通过泄殖腔按摩采集精液可获得满意效果，从而可将其用于生殖生物技术和精子冷冻保存。
