从一名21岁的X连锁肾上腺脑白质营养不良（X-ALD）患者身上诱导产生多能干细胞（iPSC）系。
Generation of induced pluripotent stem cell (iPSC) line from a 21-year-old X-linked adrenoleukodystrophy (X-ALD) patient.
作者信息
You Young Rang, Son Daryeon, Kang Phil Jun, You Seungkwon, Kim Dae-Sung
机构信息
Laboratory of Reprogramming & Differentiation, Department of Biotechnology, College of Life Science and Biotechnology, Korea University, Seoul 136-701, Republic of Korea.
Laboratory of Cell Function Regulation, Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul 136-701, Republic of Korea.
出版信息
Stem Cell Res. 2017 Dec;25:136-138. doi: 10.1016/j.scr.2017.10.016. Epub 2017 Nov 10.
X-linked Adrenoleukodystrophy (X-ALD) is a genetic disease that caused by mutations in adenosine triphosphate [ATP]-binding-cassette transporter superfamily D member 1 (ABCD1) gene. We generated an induced pluripotent stem cell (iPSC) line from a 21-year-old male X-ALD patient-derived fibroblasts by Sendai virus mediated reprogramming. Established iPSCs stably expanded while maintaining immunoreactivity for various pluripotency markers and alkaline phosphatase, as well as normal 44+XY karyotype. Under the differentiation condition, the cells gave rise to cells of three germ layers.
X连锁肾上腺脑白质营养不良（X-ALD）是一种由三磷酸腺苷[ATP]结合盒转运体超家族D成员1（ABCD1）基因突变引起的遗传性疾病。我们通过仙台病毒介导的重编程，从一名21岁男性X-ALD患者的成纤维细胞中生成了诱导多能干细胞（iPSC）系。建立的iPSC稳定扩增，同时对各种多能性标志物和碱性磷酸酶保持免疫反应性，以及正常的44+XY核型。在分化条件下，这些细胞产生了三个胚层的细胞。
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