通过蛋白质组学鉴定洋葱伯克霍尔德菌中由AHL和BDSF调控的蛋白质
Identification of AHL- and BDSF-Controlled Proteins in Burkholderia cenocepacia by Proteomics.
作者信息
Liu Yilei, Pessi Gabriella, Riedel Katharina, Eberl Leo
机构信息
Department of Plant and Microbial Biology, University of Zurich, Zollikerstrasse 107, 8008, Zurich, Switzerland.
Institute of Microbiology, University of Greifswald, Greifswald, Germany.
出版信息
Methods Mol Biol. 2018;1673:193-202. doi: 10.1007/978-1-4939-7309-5_15.
We used comparative proteome analysis to determine the target genes of the two quorum sensing (QS) circuits in the opportunistic pathogen Burkholderia cenocepacia: the N-acyl homoserine lactone (AHL)-based CepIR system and the BDSF (B urkholderia diffusible signal factor, cis-2-dodecenoic acid)-based RpfFR system. In this book chapter, we focus on the description of the practical procedure we currently use in the laboratory to perform a sensitive GeLC-MS/MS shotgun proteomics experiment; we also briefly describe the downstream bioinformatic data analysis.
我们采用比较蛋白质组分析方法，以确定机会致病菌洋葱伯克霍尔德菌中两个群体感应（QS）系统的靶基因：基于N-酰基高丝氨酸内酯（AHL）的CepIR系统和基于BDSF（伯克霍尔德菌可扩散信号因子，顺式-2-十二碳烯酸）的RpfFR系统。在本章中，我们重点描述了目前实验室用于进行灵敏的凝胶液相色谱-串联质谱鸟枪法蛋白质组学实验的实际操作流程；我们还简要介绍了下游的生物信息学数据分析。
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