研究慢性乙型肝炎病毒感染者体内乙型肝炎病毒自发清除过程中 CD8+记忆 T 细胞亚群分布及 IFN-γ水平的变化。

Study on the distribution of CD8+ memory T cell subsets and IFN-γ level during the spontaneous clearance of hepatitis B virus in patients with chronic hepatitis B virus infection.

机构信息

Department of Infectious Diseases, Guangdong General Hospital, Guangdong Academy of Medical Sciences, Guangdong, China.

出版信息

Eur Rev Med Pharmacol Sci. 2017 Oct;21(20):4675-4679.

Abstract

OBJECTIVE

To study the alteration of CD8+ memory T cell subsets under different immune statuses during the spontaneous clearance of hepatitis B virus (HBV) in Chinese patients with chronic HBV infection.

PATIENTS AND METHODS

We analyzed Chinese patients with chronic HBV infection including 10 patients with Hepatitis B surface Antigen (HBsAg) spontaneous seroconversion, 25 patients with Hepatitis B virus e Antigen (HBeAg) spontaneous seroconversion, 25 patients with chronic hepatitis B (CHB), and 25 chronic HBV carriers. The CD8+ T cells in peripheral blood were isolated, and flow cytometry was used to determine the percent change of CD8+ T memory cell subsets. ELISA was used to measure the levels of Interferon-γ (IFN-γ) secretion from CD8+ T cells.

RESULTS

(1) The percentage of CD8+ TN cells in peripheral blood was lower in the HBsAg seroconversion group than in the HBeAg seroconversion group (p<0.01), and higher in the CHB group and chronic HBV carrier group (p<0.01, 0.01); (2) The percentage of CD8+ TEM-2 memory T cells in peripheral blood was higher in the HBsAg seroconversion group than the HBeAg seroconversion group (p<0.05), CHB group, and chronic HBV carrier group (p<0.01, 0.01); (3) The percentage of CD8+ TEM-1 and CD8+ TCM cells in peripheral blood was higher in the CHB group and HBV carrier group than the HBsAg seroconversion group and HBeAg group, but there were no significant differences between groups (p>0.05); (4) IFN-γ production from CD8+ T cells in peripheral blood was higher in the HBsAg seroconversion group than the HBeAg seroconversion group (p<0.05), CHB group, and chronic HBV carrier group (p<0.05, 0.01).

CONCLUSIONS

The consistent increase of CD8+ TEM-2 cell subsets may be an important cause of spontaneous clearance of HBV. The disorder of CD8+ memory T cell differentiation may be an important mechanism of chronic HBV infection.

摘要

目的

研究中国慢性乙型肝炎病毒（HBV）感染者在 HBV 自发清除过程中不同免疫状态下 CD8+记忆 T 细胞亚群的变化。

方法

分析包括 10 例 HBsAg 自发血清学转换、25 例 HBeAg 自发血清学转换、25 例慢性乙型肝炎（CHB）和 25 例慢性 HBV 携带者在内的中国慢性 HBV 感染者。分离外周血 CD8+T 细胞，采用流式细胞术测定 CD8+T 记忆细胞亚群的百分比变化，采用 ELISA 法测定 CD8+T 细胞干扰素-γ（IFN-γ）分泌水平。

结果

（1）HBsAg 血清学转换组外周血 CD8+TN 细胞百分比低于 HBeAg 血清学转换组（p<0.01），高于 CHB 组和慢性 HBV 携带者组（p<0.01，0.01）；（2）HBsAg 血清学转换组外周血 CD8+TEM-2 记忆 T 细胞百分比高于 HBeAg 血清学转换组（p<0.05）、CHB 组和慢性 HBV 携带者组（p<0.01，0.01）；（3）CHB 组和慢性 HBV 携带者组外周血 CD8+TEM-1 和 CD8+TCM 细胞百分比高于 HBsAg 血清学转换组和 HBeAg 组，但组间无显著差异（p>0.05）；（4）HBsAg 血清学转换组外周血 CD8+T 细胞 IFN-γ的产生高于 HBeAg 血清学转换组（p<0.05）、CHB 组和慢性 HBV 携带者组（p<0.05，0.01）。