Sprynskyy Myroslav, Krzemień-Konieczka Iwona, Gadzała-Kopciuch Renata, Buszewski Bogusław
Department of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, 7 Gagarina Str., 87-100 Toruń, Poland.
Department of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, 7 Gagarina Str., 87-100 Toruń, Poland.
J Chromatogr B Analyt Technol Biomed Life Sci. 2018 Jan 1;1072:1-8. doi: 10.1016/j.jchromb.2017.11.001. Epub 2017 Nov 4.
The objective of the study was to examine adsorption of the aflatoxin B1 from synthetic gastric fluid and synthetic intestinal fluid by talc, raw and calcined diatomite. The kinetic and equilibrium adsorption processes were studied in the batch adsorption experiments applying high performance liquid chromatography for the aflatoxin B1 determination. The kinetic study showed a very fast adsorption of the aflatoxin B1 onto the selected adsorbents from the both physiological fluids with reaching equilibrium within 1-15min. The aflatoxin B1 was almost completely adsorbed in initial linear step of the kinetic process that can be described well by the zero-order kinetics model. The experimental data of the equilibrium adsorption were characterized using the Langmuir and Freundlich isotherm models. The high adsorption effectiveness was found in a range of 90%-100% and 60%-100% for the diatomite samples and the talc respectively at the initial concentrations of the aflatoxin B1 as 31-300ng/mL. The possible mechanisms of the aflatoxin adsorption onto the used mineral adsorbents are also discussed in the work.
该研究的目的是考察滑石粉、生硅藻土和煅烧硅藻土对合成胃液和合成肠液中黄曲霉毒素B1的吸附情况。在批量吸附实验中，采用高效液相色谱法测定黄曲霉毒素B1，研究了其动力学和平衡吸附过程。动力学研究表明，黄曲霉毒素B1从两种生理流体中非常快速地吸附到所选吸附剂上，在1-15分钟内达到平衡。黄曲霉毒素B1在动力学过程的初始线性阶段几乎被完全吸附，可用零级动力学模型很好地描述。使用朗缪尔和弗伦德里希等温线模型对平衡吸附的实验数据进行了表征。在黄曲霉毒素B1初始浓度为31-300ng/mL时，硅藻土样品和滑石粉的吸附效率分别在90%-100%和60%-100%范围内。该研究还讨论了黄曲霉毒素吸附到所用矿物吸附剂上的可能机制。
