Faculdade de Ciências Médicas, University of Campinas, Campinas, SP, Brazil.
Instituto de Biologia, University of Campinas, Campinas, SP, Brazil.
BMC Cancer. 2017 Nov 13;17(1):756. doi: 10.1186/s12885-017-3716-4.
The spontaneous immortalization of primary malignant cells is frequently assigned to their genetic instability during in vitro culturing. In this study, the new epithelial ovarian cancer cell line CAISMOV24 was described and compared with its original low-grade serous ovarian carcinoma.
The in vitro culture was established with cells isolated from ascites of a 60-year-old female patient with recurrent ovarian cancer. The CAISMOV24 line was assessed for cell growth, production of soluble biomarkers, expression of surface molecules and screened for typical mutations found in serous ovarian carcinoma. Additionally, comparative genomic hybridization was employed to compare genomic alterations between the CAISMOV24 cell line and its primary malignant cells.
CAISMOV24 has been in continuous culture for more than 30 months and more than 100 in vitro passages. The cell surface molecules EpCAM, PVR and CD73 are overexpressed on CAISMOV24 cells compared to the primary malignant cells. CAISMOV24 continues to produce CA125 and HE4 in vitro. Although the cell line had developed alongside the accumulation of genomic alterations (28 CNV in primary cells and 37 CNV in CAISMOV24), most of them were related to CNVs already present in primary malignant cells. CAISMOV24 cell line harbored KRAS mutation with wild type TP53, therefore it is characterized as low-grade serous carcinoma.
Our results corroborate with the idea that genomic alterations, depicted by CNVs, can be used for subtyping epithelial ovarian carcinomas. Additionally, CAISMOV24 cell line was characterized as a low-grade serous ovarian carcinoma, which still resembles its primary malignant cells.
原发性恶性细胞的自发永生化通常归因于其在体外培养过程中的遗传不稳定性。本研究描述了一种新的卵巢上皮癌细胞系 CAISMOV24，并将其与原始的低级别浆液性卵巢癌进行了比较。
从一名 60 岁女性复发性卵巢癌患者的腹水分离细胞，建立体外培养。评估 CAISMOV24 系的细胞生长、可溶性生物标志物的产生、表面分子的表达，并筛选出浆液性卵巢癌中常见的突变。此外，还采用比较基因组杂交技术比较 CAISMOV24 细胞系与其原始恶性细胞的基因组改变。
CAISMOV24 已连续培养超过 30 个月和 100 多次体外传代。与原始恶性细胞相比，CAISMOV24 细胞表面分子 EpCAM、PVR 和 CD73 过表达。CAISMOV24 继续在体外产生 CA125 和 HE4。尽管该细胞系伴随着基因组改变的积累（原发性细胞中有 28 个 CNV，CAISMOV24 中有 37 个 CNV），但大多数改变与原发性恶性细胞中已经存在的 CNV 相关。CAISMOV24 细胞系存在 KRAS 突变和野生型 TP53，因此其特征为低级别浆液性癌。
我们的结果证实了基因组改变（由 CNV 描述）可用于上皮性卵巢癌的亚分型。此外，CAISMOV24 细胞系被鉴定为低级别浆液性卵巢癌，其仍与原始恶性细胞相似。
