Agatonovic-Kustrin S, Morton D W, Adam A, Mizaton H H, Zakaria H
School of Pharmacy, Monash University Malaysia, Jalan Lagoon Selatan, Bandar Sunway, 47500, Selangor Darul Ehsan, Malaysia; School of Pharmacy and Applied Science, La Trobe Institute for Molecular Sciences, La Trobe University, Edwards Rd, Bendigo, 3550, Australia; Faculty of Pharmacy, Universiti Teknologi MARA, Puncak Alam Campus, Bandar Puncak Alam, Selangor, 42300, Malaysia.
School of Pharmacy and Applied Science, La Trobe Institute for Molecular Sciences, La Trobe University, Edwards Rd, Bendigo, 3550, Australia; Faculty of Pharmacy, Universiti Teknologi MARA, Puncak Alam Campus, Bandar Puncak Alam, Selangor, 42300, Malaysia.
J Chromatogr A. 2017 Dec 29;1530:192-196. doi: 10.1016/j.chroma.2017.11.012. Epub 2017 Nov 10.
The steady increase of diabetes is becoming a major burden on health care systems. As diabetic complications arise from oxidative stress, an antioxidant therapy along with anti-diabetic drugs is recommended. Myrmecodia or ant plant is highly valued as a traditional medicine in West Papua. It is used as an alternative treatment for diabetes, as the substances produced by ants can reduce blood sugar levels. The aim of this study was to develop and establish high-performance thin-layer chromatographic (HPTLC)-bioautographic methods to measure the antioxidant and hypoglycemic effects in different extracts from Myrmecodia platytyrea and to compare them with sterol content. Antioxidant activity in methanol, ethanol, dichloromethane (DCM) and ethyl acetate (EA) extracts were measured with a direct HPTLC-2,2-diphenyl-1-picrylhydrazyl free radical (DPPH) assay, while hypoglycemic effects were assessed using a newly developed α-amylase inhibitory activity assay. Stigmasterol is observed, after derivatization with anisaldehyde, as purple colored zones under visible light at hRF values of 0.66. The highest antioxidant activity was observed in the ethanol extract which is rich in polyphenols and flavonoids, while the DCM extract did not show antioxidant activity, but had significant α-amylase inhibitory activity. The highest α-amylase inhibitory activity was observed in the EA and DCM extracts and was related to their stigmasterol content.
糖尿病发病率的稳步上升正成为医疗保健系统的一大负担。由于糖尿病并发症源于氧化应激，因此建议在使用抗糖尿病药物的同时进行抗氧化治疗。蚁巢木属植物或蚁栖植物在西巴布亚作为传统药物备受重视。它被用作糖尿病的替代治疗方法，因为蚂蚁产生的物质可以降低血糖水平。本研究的目的是开发并建立高效薄层色谱（HPTLC）-生物自显影方法，以测定宽叶蚁巢木不同提取物中的抗氧化和降血糖作用，并将其与甾醇含量进行比较。采用直接HPTLC-2,2-二苯基-1-苦基肼自由基（DPPH）法测定甲醇、乙醇、二氯甲烷（DCM）和乙酸乙酯（EA）提取物中的抗氧化活性，同时使用新开发的α-淀粉酶抑制活性测定法评估降血糖作用。用茴香醛衍生化后，在可见光下观察到豆甾醇在hRF值为0.66时呈现紫色区域。在富含多酚和黄酮类化合物的乙醇提取物中观察到最高的抗氧化活性，而DCM提取物未显示抗氧化活性，但具有显著的α-淀粉酶抑制活性。在EA和DCM提取物中观察到最高的α-淀粉酶抑制活性，这与它们的豆甾醇含量有关。
