Suzuki Yoshihiro, Niina Kouki, Matsuwaki Tomonori, Nukazawa Kei, Iguchi Atsushi
a Department of Civil and Environmental Engineering , Faculty of Engineering, University of Miyazaki , Miyazaki , Japan.
b Department of Animal and Grassland Sciences , Faculty of Agriculture, University of Miyazaki , Miyazaki , Japan.
J Environ Sci Health A Tox Hazard Subst Environ Eng. 2018 Jan 28;53(2):160-173. doi: 10.1080/10934529.2017.1383128. Epub 2017 Nov 17.
The aim of this study was to rapidly and effectively analyze coliforms, which are the most fundamental indicators of water quality for fecal pollution, using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). Coliform bacteria were isolated from municipal sewage, river water, and groundwater. For each sample, 100 isolates were determined by MALDI-TOF MS. In addition, these same 100 isolates were also identified via 16S rRNA gene sequence analysis. Obtained MALDI-TOF MS data were compared with the 16S rRNA sequencing analysis, and the validity of MALDI-TOF MS for classification of coliform bacteria was examined. The concordance rate of bacterial identification for the 100 isolates obtained by MALDI-TOF MS analysis and 16S rRNA gene sequence analysis for sewage, river water, and ground water were 96%, 74%, and 62% at the genus level, respectively. Among the sewage, river water, and ground water samples, the coliform bacterial flora were distinct. The dominant genus of coliforms in sewage, river water, and groundwater were Klebsiella spp., Enterobacter spp., and Serratia spp., respectively. We determined that MALDI-TOF MS is a rapid and accurate tool that can be used to identify coliforms. Therefore, without using conventional 16S rRNA sequencing, it is possible to rapidly and effectively classify coliforms in water using MALDI-TOF MS.
本研究的目的是使用基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）快速有效地分析大肠菌群，大肠菌群是粪便污染水质的最基本指标。从城市污水、河水和地下水中分离出大肠菌群。对于每个样本，通过MALDI-TOF MS鉴定100株分离菌。此外，这100株分离菌也通过16S rRNA基因序列分析进行鉴定。将获得的MALDI-TOF MS数据与16S rRNA测序分析结果进行比较，检验MALDI-TOF MS对大肠菌群分类的有效性。通过MALDI-TOF MS分析和16S rRNA基因序列分析获得的100株污水、河水和地下水分离菌的细菌鉴定一致率在属水平分别为96%、74%和62%。在污水、河水和地下水样本中，大肠菌群的菌群不同。污水、河水和地下水中大肠菌群的优势属分别为克雷伯菌属、肠杆菌属和沙雷菌属。我们确定MALDI-TOF MS是一种可用于鉴定大肠菌群的快速准确工具。因此，无需使用传统的16S rRNA测序，使用MALDI-TOF MS就可以快速有效地对水中的大肠菌群进行分类。
