Okwudili Onoja Samuel, Chimaobi Nwachukwu Glory, Ikechukwu Ezeja Maxwell, Ndukaku Omeh Yusuf
Department of Veterinary Physiology, Michael Okpara University of Agriculture Umudike, Pharmacology, Biochemistry and Animal Health and Production, College of Veterinary Medicine, PMB 7267, Abia State Umuahia, Nigeria.
Department of Biochemistry, Michael Okpara University of Agriculture, College of Natural Sciences, PMB 7267, Abia State Umuahia, Nigeria.
J Complement Integr Med. 2017 Nov 17;15(2):/j/jcim.2018.15.issue-2/jcim-2015-0017/jcim-2015-0017.xml. doi: 10.1515/jcim-2015-0017.
Background The study evaluated phytochemical composition, antidiabetic, oral glucose tolerance test and in vitro antioxidant activities of hydromethanol extract of Paullinia pinnata root bark. Methods Cold maceration method was used in extract preparation and scavenging of 2,2-diphenyl-1-picrylhydrazyl radicals was used to evaluate antioxidant properties of the extract. Diabetes was induced with alloxan at the dose of 160 mg/kg. The antidiabetic activity of the extract was tested at doses of 50, 100 and 200 mg/kg, and glibenclamide was used as reference drug. Results Phytochemical analysis of the extract showed the presence of alkaloids, flavonoids, glycosides, tannins, saponins and terpenes/sterols. The extract produced a significant (p<0.05) time-dependent decrease in the fasting blood glucose (FBG) levels in the treated rats when compared with the distilled water treated rats, but did not produce dose-dependent effects. The extract 50, 100 and 200 mg/kg and glibenclamide (2 mg/kg) caused 83.62 %, 60.66 %, 47.77 % and 68.52 % reduction respectively in FBG at 6 h post-treatment while the distilled water (5 mL/kg) produced 8.12 % reduction in FBG at 6 h post treatment. The extract (50 mg/kg) and glibenclamide (2 mg/kg) produced a significant (p<0.05) oral glucose tolerance effect in both normoglycemic and diabetic rats. The extract produced concentration-dependent increase in antioxidant activity and had its optimum effect at 400 µg/mL concentration. Conclusions This study suggests that P. pinnata root bark has potent antidiabetic and antioxidant activities and also validates its use in folkloric medicine in the management of diabetes-related conditions.
本研究评估了羽叶泡林藤根皮水甲醇提取物的植物化学成分、抗糖尿病作用、口服葡萄糖耐量试验及体外抗氧化活性。方法：采用冷浸法制备提取物，通过清除2,2-二苯基-1-苦基肼自由基来评估提取物的抗氧化性能。用剂量为160mg/kg的四氧嘧啶诱导糖尿病。以50、100和200mg/kg的剂量测试提取物的抗糖尿病活性，格列本脲用作参比药物。结果：提取物的植物化学分析表明存在生物碱、黄酮类、糖苷、单宁、皂苷和萜类/甾醇。与蒸馏水处理的大鼠相比，提取物使治疗大鼠的空腹血糖（FBG）水平出现显著（p<0.05）的时间依赖性降低，但未产生剂量依赖性效应。提取物50、100和200mg/kg以及格列本脲（2mg/kg）在治疗后6小时分别使FBG降低83.62%、60.66%、47.77%和68.52%，而蒸馏水（5mL/kg）在治疗后6小时使FBG降低8.12%。提取物（50mg/kg）和格列本脲（2mg/kg）在正常血糖和糖尿病大鼠中均产生显著（p<0.05）的口服葡萄糖耐量效应。提取物产生浓度依赖性的抗氧化活性增加，在400μg/mL浓度时具有最佳效果。结论：本研究表明羽叶泡林藤根皮具有强大的抗糖尿病和抗氧化活性，也证实了其在民间医学中用于管理糖尿病相关病症的用途。
