Marrero-Rodríguez Daniel, la Cruz Hugo Arreola-De, Taniguchi-Ponciano Keiko, Gomez-Virgilio Laura, Huerta-Padilla Victor, Ponce-Navarrete Gustavo, Andonegui-Elguera Sergio, Jimenez-Vega Florinda, Romero-Morelos Pablo, Rodriguez-Esquivel Miriam, Meraz-Rios Marco, Figueroa-Corona Ma Del Pilar, Monroy Alberto, Pérez-González Oscar, Salcedo Mauricio
Laboratorio de Oncología Genómica, Unidad de Investigación Médica en Enfermedades Oncológicas, Hospital de Oncología, CMN-SXXI, Instituto Mexicano del Seguro Social, Ciudad de México, México; Departamento de Biomedicina Molecular, Centro de Investigación y de Estudios Avanzados, Ciudad de México, México.
Laboratorio de Oncología Genómica, Unidad de Investigación Médica en Enfermedades Oncológicas, Hospital de Oncología, CMN-SXXI, Instituto Mexicano del Seguro Social, Ciudad de México, México.
Arch Med Res. 2017 May;48(4):314-322. doi: 10.1016/j.arcmed.2017.06.011.
Krüppel Like Factors (KLF) refers to a family of seventeen members of transcription factors. Involved in several cellular processes. As other cancer types, Cervical Cancer (CC) presents molecular deregulations in transcription factors, but especially Human Papilloma Virus (HPV) sequences. Here in this work we analyzed the mRNA expression of all KLF family members in CC-derived cell lines and CC tissues.
The cell lines used were HeLa, INBL, RoVa, C4-I, Ms751, ViPa, CaLo, SiHa, CaSki, C33a and ViBo and the non-tumorigenic HaCaT. mRNA expression was analyzed by means of expression microarray and RT-PCR, and KLF5 protein by immunofluorescence.
The cell lines were grouped according to HPV genotype as HPV16, HPV18 positive or HPV negative cells. Heterogeneous expression was observed among the cell lines. Despite the heterogeneous expression profile, KLF3, -5, -12, -15 and -16 transcripts were present in all cell lines, KLF4 and -10 which were not expressed in CaSki; KLF11 and 13 were not expressed by Vipa and C4-I, and KLF7 was not expressed by C4-I and Rova. The CC tissue analysis shows expression of most of the KLF members, such as KLF5. KLF5 immunosignal was positive in the three cell lines analyzed.
We suggest that KLF expression could not be related to HPV presence/genotype, at least at transcriptional level, and the expression of KLF family members may be necessary in the biology of the CC cells.
Krüppel样因子（KLF）是指一个由17个转录因子成员组成的家族，参与多种细胞过程。与其他癌症类型一样，宫颈癌（CC）在转录因子方面存在分子失调，尤其是人乳头瘤病毒（HPV）序列。在本研究中，我们分析了CC衍生细胞系和CC组织中所有KLF家族成员的mRNA表达。
使用的细胞系包括HeLa、INBL、RoVa、C4-I、Ms751、ViPa、CaLo、SiHa、CaSki、C33a和ViBo以及非致瘤性HaCaT。通过表达微阵列和逆转录-聚合酶链反应（RT-PCR）分析mRNA表达，并通过免疫荧光分析KLF5蛋白。
细胞系根据HPV基因型分为HPV16、HPV18阳性或HPV阴性细胞。在细胞系中观察到异质性表达。尽管表达谱存在异质性，但所有细胞系中均存在KLF3、-5、-l2、-l5和-16转录本，CaSki中未表达KLF4和-10；Vipa和C4-I未表达KLF11和13，C4-I和RoVa未表达KLF7。CC组织分析显示大多数KLF成员有表达，如KLF5。在分析的三个细胞系中，KLF5免疫信号为阳性。
我们认为，至少在转录水平上，KLF表达可能与HPV的存在/基因型无关，KLF家族成员的表达可能是CC细胞生物学所必需的。
