赞比亚血清反应阳性母亲中乳铁传递蛋白基因多态性与HIV-1传播风险的关联
Association Between LTF Polymorphism and Risk of HIV-1 Transmission Among Zambian Seropositive Mothers.
作者信息
Zupin Luisa, Polesello Vania, Segat Ludovica, Kamada Anselmo Jiro, Kuhn Louise, Crovella Sergio
机构信息
Department of Medicine, Surgery and Health Sciences, University of Trieste, Trieste, Italy.
Institute for Maternal and Child Health IRCCS "Burlo Garofolo", Trieste, Italy.
出版信息
Curr HIV Res. 2018;16(1):52-57. doi: 10.2174/1570162X15666171120105752.
BACKGROUND
Lactoferrin is a member of the innate immune system acting in the first line of defence against pathogens, and it is known for its antibacterial, antifungal and antiviral activity, including HIV-1. Two polymorphisms, T29A and R47K, in the exon 1 region of the LTF gene (encoding for the lactoferrin protein) were previously described as able to influence the lactoferrin antimicrobial function.
OBJECTIVES
LTF T29A and R47K genetic variants were analysed in a Zambian population to unravel if these polymorphisms could play a role in HIV-1 mother-to-child HIV-1 transmission.
METHODS
LTF T29A and R47K polymorphisms were genotyped, using allelic specific fluorescent probes and real time PCR, in a population comprising 101 HIV-1 positive mothers and 333 children born to seropositive mothers.
RESULTS
Maternal LTF T29A A/A and A/G genotypes were found to be associated with decreased risk of HIV-1 MTCT, being more frequent among non-transmitter mothers respect to transmitter mothers.
CONCLUSION
Our data suggested that maternal LTF genetic background contributes to the susceptibility to HIV-1 transmission from mother to new-borns.
背景
乳铁蛋白是先天性免疫系统的成员，在抵御病原体的第一道防线中发挥作用，并且以其抗菌、抗真菌和抗病毒活性（包括对HIV-1的活性）而闻名。LTF基因（编码乳铁蛋白）外显子1区域中的两个多态性T29A和R47K，先前被描述为能够影响乳铁蛋白的抗菌功能。
目的
在赞比亚人群中分析LTF T29A和R47K基因变异，以阐明这些多态性是否可能在HIV-1母婴传播中起作用。
方法
使用等位基因特异性荧光探针和实时PCR，对由101名HIV-1阳性母亲和333名血清阳性母亲所生子女组成的人群进行LTF T29A和R47K多态性基因分型。
结果
发现母亲的LTF T29A A/A和A/G基因型与HIV-1母婴传播风险降低相关，在未传播母亲中比传播母亲中更常见。
结论
我们的数据表明，母亲的LTF基因背景有助于母亲向新生儿传播HIV-1的易感性。
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