Gerrard J M, Robinson P
Department of Pediatrics, University of Manitoba, Winnipeg, Canada.
Biochim Biophys Acta. 1989 Feb 20;1001(3):282-5. doi: 10.1016/0005-2760(89)90112-4.
Platelets, when stirred with 3 U thrombin/10(9) platelets, produced significant quantities of palmitoyllysophosphatidic acid (2.17 ng/10(9) platelets), stearoyllysophosphatidic acid (2.11 ng/10(9) platelets), and arachidonoyllysophosphatidic acid (1.06 ng/10(9) platelets). When platelets were pretreated with 100 microM of the phospholipase A2 inhibitor U10029A, there was a significant decrease in thrombin-stimulated production of stearoyllysophosphatidic acid (to 0.16 ng/10(9) platelets), while arachidonoyllysophosphatidic acid production was unchanged. U10029A concomitantly increased thrombin-stimulated production of stearoyl-containing phosphatidic acid species (primarily stearoylarachidonoylphosphatidic acid) from 5.99 to 9.71 ng/10(9) platelets. The results are consistent with the concept that stearoyllysophosphatidic acid production in platelets occurs via phospholipase A2 degradation of phosphatidic acid.
血小板在与3 U凝血酶/10⁹个血小板一起搅拌时，会产生大量的棕榈酰溶血磷脂酸（2.17 ng/10⁹个血小板）、硬脂酰溶血磷脂酸（2.11 ng/10⁹个血小板）和花生四烯酰溶血磷脂酸（1.06 ng/10⁹个血小板）。当血小板用100 μM的磷脂酶A2抑制剂U10029A预处理时，凝血酶刺激产生的硬脂酰溶血磷脂酸显著减少（降至0.16 ng/10⁹个血小板），而花生四烯酰溶血磷脂酸的产生没有变化。U10029A同时使凝血酶刺激产生的含硬脂酰的磷脂酸种类（主要是硬脂酰花生四烯酰磷脂酸）从5.99 ng/10⁹个血小板增加到9.71 ng/10⁹个血小板。这些结果与血小板中硬脂酰溶血磷脂酸的产生是通过磷脂酸的磷脂酶A2降解这一概念一致。
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