Kim H W, Lee K, Kim S H, Rhee M S
Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul 02841, Republic of Korea.
Food Microbiology Division, National Institute of Food and Drug Safety Evaluation, North Chungcheong Province 28159, Republic of Korea.
Food Microbiol. 2018 Apr;70:129-136. doi: 10.1016/j.fm.2017.09.017. Epub 2017 Oct 5.
The objectives of the current study were to investigate the fate of microbial indicators [aerobic plate counts (APC), total coliforms (TC), and lactic acid bacteria (LAB)] in commercial salted napa cabbages during storage conditions at different temperatures (5, 22, and 30 °C, for up to 72 h) and to develop a predictive growth model using the modified Gompertz equation to determine shelf life. Microbial population sizes (initial log CFU g: APC, 5.1; TC, 3.0; LAB, 3.7) remained stable at 5 °C, but rapidly increased by 2-4 log CFU g within 12 h at 22 and 30 °C; furthermore, the pH of salted napa cabbages decreased significantly (P < 0.05: initial pH 6.3; final pH 4.1-4.4) due to LAB fermentation. The pH showed a negative correlation with all bacterial groups and did not prevent the growth of TC during storage. According to the modified Gompertz model (R ≧ 0.97), the highest μ was observed for LAB at 30 °C [0.61 log CFU h], while the lowest was noted for TC at 5 °C [0.04 log CFU h]. Shelf-life was determined using APC (7.7 log CFU g) and LAB (6.0 log CFU g) limits; the microbiological acceptability period of salted napa cabbage was predicted to be 12.6 and 9.3 h at 22 and 30 °C, respectively. Thus, consumers should use the product within 12 h of storage at room temperature (more quickly in the summer (9 h)), or store it in a refrigerator. The presented research proposes a shelf-life modeling of commercial salted napa cabbages, which may be used as a scientific basis for product quality control and issuing appropriate guidance for consumer use at home.
本研究的目的是调查市售盐渍大白菜在不同温度(5、22和30℃,长达72小时)储存条件下微生物指标[需氧平板计数(APC)、总大肠菌群(TC)和乳酸菌(LAB)]的变化情况,并使用修正的Gompertz方程建立预测生长模型以确定保质期。微生物种群数量(初始对数CFU/g:APC为5.1;TC为3.0;LAB为3.7)在5℃时保持稳定,但在22和30℃下12小时内迅速增加2-4对数CFU/g;此外,由于LAB发酵,盐渍大白菜的pH值显著降低(P<0.05:初始pH值6.3;最终pH值4.1-4.4)。pH值与所有细菌组呈负相关,且在储存期间并未阻止TC的生长。根据修正的Gompertz模型(R≥0.97),LAB在30℃时的最大生长速率(μ)最高[0.61对数CFU/h],而TC在5℃时的最低[0.04对数CFU/h]。使用APC(7.7对数CFU/g)和LAB(6.0对数CFU/g)的限量来确定保质期;预测盐渍大白菜在22和30℃下的微生物可接受期分别为12.6和9.3小时。因此,消费者应在室温下储存12小时内使用该产品(夏季更快(9小时)),或冷藏保存。本研究提出了市售盐渍大白菜的保质期模型,可为产品质量控制提供科学依据,并为消费者在家中使用提供适当指导。