Hirota N, Williams G M
J Natl Cancer Inst. 1979 Nov;63(5):1257-65.
Altered (hyperplastic) foci and neoplastic (hyperplastic) nodules identified by their resistance to iron accumulation were induced in the livers of F344 rats by limited feeding of N-2-fluorenylacetamide for three, four, or five cycles of 4-week intervals separated by 1 week of a basal diet. Foci were present by the end of three feeding cycles and increased in number with further carcinogen exposure. No nodules were present at the end of three or four cycles, but they appeared at later intervals after removal of the carcinogen. Nodules were present at the end of five cycles of feeding and increased in number later. Thus nodules were found to be persistent and to have the progressive growth ability in situ that is characteristic of neoplasms.
通过给F344大鼠限量喂食N-2-芴基乙酰胺，间隔4周进行三个、四个或五个周期的喂食，每个周期中间有1周的基础饮食期，从而在其肝脏中诱导出对铁积累有抗性的改变（增生）灶和肿瘤性（增生）结节。三个喂食周期结束时出现了病灶，随着进一步接触致癌物，病灶数量增加。三个或四个周期结束时没有结节，但在去除致癌物后的较晚时间间隔出现了结节。五个喂食周期结束时出现了结节，随后数量增加。因此，发现结节具有持久性，并且在原位具有肿瘤特有的渐进性生长能力。
