一种基于荧光的细菌拓扑异构酶I抑制剂鉴定分析方法。
A Fluorescence-Based Assay for Identification of Bacterial Topoisomerase I Poisons.
作者信息
Annamalai Thirunavukkarasu, Cheng Bokun, Keswani Neelam, Tse-Dinh Yuk-Ching
机构信息
Department of Chemistry and Biochemistry, Florida International University, Miami, FL, USA.
Biomolecular Sciences Institute, Florida International University, Miami, FL, USA.
出版信息
Methods Mol Biol. 2018;1703:259-268. doi: 10.1007/978-1-4939-7459-7_18.
Bacterial Topoisomerase I is a potential target for the identification of novel topoisomerase poison inhibitors that could provide leads for a new class of antibacterial compounds. Here we describe in detail a fluorescence-based cleavage assay that is successfully used in HTS for the discovery of bacterial topoisomerase Ι poisons.
细菌拓扑异构酶I是鉴定新型拓扑异构酶中毒抑制剂的潜在靶点，这些抑制剂可为新型抗菌化合物提供线索。在此，我们详细描述了一种基于荧光的切割测定法，该方法已成功用于高通量筛选以发现细菌拓扑异构酶I中毒剂。
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