酪醇对在不同表面形成的白色念珠菌、光滑念珠菌和变形链球菌生物膜的作用。

Role of tyrosol on Candida albicans, Candida glabrata and Streptococcus mutans biofilms developed on different surfaces.

作者信息

Monteiro Douglas Roberto, Arias Laís Salomão, Fernandes Renan Aparecido, Straioto Fabiana Gouveia, Barros Barbosa Débora, Pessan Juliano Pelim, Delbem Alberto Carlos Botazzo

机构信息

Department of Pediatric Dentistry and Public Health, São Paulo State University (Unesp), School of Dentistry, Araçatuba, São Paulo, Brazil.

Assistant Professor, Department of Prosthodontics, Universidade do Oeste Paulista (UNOESTE), Presidente Prudente, São Paulo, Brazil.

出版信息

Am J Dent. 2017 Feb;30(1):35-39.

PMID:29178712

Abstract

PURPOSE

To assess the effect of tyrosol on the production of hydrolytic enzymes (by Candida biofilm cells) and acid (by Streptococcus mutans biofilms), as well as to quantify single and mixed biofilms of these species formed on acrylic resin (AR) and hydroxyapatite (HA).

METHODS

Candida and S. mutans biofilms were formed on AR and HA in the presence of tyrosol during 48 hours. Next, acid proteinase, phospholipase and hemolytic activities of Candida biofilm cells were determined, while acid production by S. mutans biofilms was assessed by pH determination. The effect of tyrosol on mature biofilms (96 hours) was evaluated through quantification of total biomass, metabolic activity, number of colony-forming units and composition of biofilms' extracellular matrix. Data were analyzed by one- and two-way ANOVA, followed by Tukey's and Holm-Sidak's tests (α = 0.05).

RESULTS

Treatments with tyrosol were not able to significantly reduce hydrolytic enzymes and acid production by Candida and S. mutans. Tyrosol only significantly reduced the metabolic activity of single biofilms of Candida species.

CLINICAL SIGNIFICANCE

Tyrosol on its own had a limited efficacy against single and mixed-species oral biofilms. Its use as an alternative antimicrobial for topical therapies still demands more investigation.

摘要

目的

评估酪醇对水解酶（由念珠菌生物膜细胞产生）和酸（由变形链球菌生物膜产生）生成的影响，以及对在丙烯酸树脂（AR）和羟基磷灰石（HA）上形成的这些菌种的单一和混合生物膜进行定量分析。

方法

在酪醇存在的情况下，念珠菌和变形链球菌生物膜在AR和HA上形成48小时。接下来，测定念珠菌生物膜细胞的酸性蛋白酶、磷脂酶和溶血活性，同时通过pH测定评估变形链球菌生物膜的产酸情况。通过对总生物量、代谢活性、菌落形成单位数量和生物膜细胞外基质组成进行定量分析，评估酪醇对成熟生物膜（96小时）的影响。数据采用单因素和双因素方差分析，随后进行Tukey检验和Holm-Sidak检验（α = 0.05）。