Dr. Panjwani Center for Molecular Medicine and Drug Research, International Center for Chemical and Biological Science, University of Karachi, Karachi, 75270, Pakistan.
National institute of Blood Diseases, Karachi, Pakistan.
Sci Rep. 2017 Nov 27;7(1):16440. doi: 10.1038/s41598-017-16699-2.
Acute myeloid leukaemia (AML) is a type of cancer affecting all ages but it is more common in adults, as compared to children. Recent advancements in proteomics and mass spectrometry tools, offer a comprehensive solution to study the molecular complexity of diseases, such as cancers. This study is focused on the proteomic profiling of AML in comparison to healthy control for which, a systematic 5D proteomic approach for the fractionation of pooled plasma samples was used. Methodology includes depletion of Top-7 abundant proteins, ZOOM-isoelectric focusing (ZOOM-IEF), two-dimensional gel electrophoresis (2-DGE), and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) analysis followed by the validation of identified biomarker proteins using enzyme linked immunosorbent assay (ELISA). Up-/down-fold changes in concentration of proteins were observed in 2-DGE of AML in comparison with the healthy control and a total of 34 proteins were identified in fractioned plasma. Among them, fifteen proteins were significantly differentiated and five proteins; SAA1, complement factor C7, ApoE, plasminogen, and ApoA1 were later verified by ELISA in individual samples, which showed that SAA1 and plasminogen could be used as potential biomarker for AML.
急性髓细胞白血病（AML）是一种影响所有年龄段的癌症，但与儿童相比，成年人更为常见。蛋白质组学和质谱工具的最新进展为研究癌症等疾病的分子复杂性提供了全面的解决方案。本研究专注于 AML 的蛋白质组学分析与健康对照相比，为此，我们使用了一种系统的 5D 蛋白质组学方法来分离组合血浆样本。该方法包括 Top-7 种丰富蛋白质的耗竭、ZOOM-等电聚焦（ZOOM-IEF）、二维凝胶电泳（2-DGE）和基质辅助激光解吸/电离质谱（MALDI-MS）分析，然后使用酶联免疫吸附测定（ELISA）验证鉴定的生物标志物蛋白。与健康对照组相比，AML 的 2-DGE 中观察到蛋白质浓度的上调/下调变化，在分离的血浆中鉴定出 34 种蛋白质。其中，15 种蛋白质存在显著差异，通过 ELISA 在个体样本中进一步验证了 5 种蛋白质；SAA1、补体因子 C7、ApoE、纤溶酶原和 ApoA1，表明 SAA1 和纤溶酶原可用作 AML 的潜在生物标志物。
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