微小RNA-1271抑制肝细胞癌的细胞增殖。
MicroRNA-1271 inhibits cellular proliferation of hepatocellular carcinoma.
作者信息
Qin Andong, Zhu Jiehua, Liu Xingxiang, Zeng Dongxiao, Gu Maolin, Lv Chun
机构信息
Institute of Liver Disease, The Fourth Hospital of Huai'an, Huaian, Jiangsu 223002, P.R. China.
Department of Laboratory Medicine, The Affiliated Hospital of Zunyi Medical College, Zunyi, Guizhou 563000, P.R. China.
出版信息
Oncol Lett. 2017 Dec;14(6):6783-6788. doi: 10.3892/ol.2017.7052. Epub 2017 Sep 25.
Hepatocellular carcinoma (HCC) is one of the most common causes of cancer-associated mortality worldwide, particularly in China. MicroRNAs (miRs) serve important roles in the pathogenesis of HCC. The present study investigated the function of miR-1271 in HCC. The miR-1271 levels were analyzed by quantitative reverse transcription polymerase chain reaction. Cells growth was examined by MTT assay. Bioinformatics algorithms from TargetScanHuman were used to predict the target genes of miR-1271. The protein level was assayed by western blotting. miR-1271 demonstrated a lower expression level in HCC tissues. Upregulation of miR-1271 suppressed the growth of HepG-2 and Huh-7 cells and induced apoptosis of cells. Forkhead box Q1 () was targeted by miR-1271. In conclusion, miR-1271 is a novel tumor suppressor that inhibits HCC proliferation and induces cellular apoptosis by targeting in HCC. The results of the present study may provide a novel therapeutic target of HCC.
肝细胞癌（HCC）是全球癌症相关死亡的最常见原因之一，在中国尤为如此。微小RNA（miR）在HCC的发病机制中发挥重要作用。本研究调查了miR-1271在HCC中的功能。通过定量逆转录聚合酶链反应分析miR-1271水平。采用MTT法检测细胞生长情况。利用TargetScanHuman的生物信息学算法预测miR-1271的靶基因。通过蛋白质印迹法检测蛋白质水平。miR-1271在HCC组织中表达水平较低。miR-1271的上调抑制了HepG-2和Huh-7细胞的生长并诱导细胞凋亡。叉头框Q1（FOXQ1）是miR-1271的靶标。总之，miR-1271是一种新型肿瘤抑制因子，通过靶向HCC中的FOXQ1抑制HCC增殖并诱导细胞凋亡。本研究结果可能为HCC提供一种新的治疗靶点。
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