在……的乳汁管细胞中MYB转录因子基因的全转录组鉴定与特征分析
Transcriptome-Wide Identification and Characterization of MYB Transcription Factor Genes in the Laticifer Cells of .
作者信息
Wang Ying, Zhan Di-Feng, Li Hui-Liang, Guo Dong, Zhu Jia-Hong, Peng Shi-Qing
机构信息
Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, China.
College of Agriculture, Hainan University, Haikou, China.
出版信息
Front Plant Sci. 2017 Nov 15;8:1974. doi: 10.3389/fpls.2017.01974. eCollection 2017.
MYB transcription factors hold vital roles in the regulation of plant secondary metabolic pathways. Laticifers in rubber trees (v) are of primary importance in natural rubber production because natural rubber is formed and stored within these structures. To understand the role of MYB transcription factors in the specialized cells, we identified 44 MYB genes (named to ) by using our previously obtained transcriptome database of rubber tree laticifer cells and the public rubber tree genome database. Expression profiles showed that five MYB genes were highly expressed in the laticifers. and were selected for further study. HblMYB19 and HblMYB44 bound the promoters of , , and in yeast. Furthermore, the transient overexpression of HblMYB19 and HblMYB44 in tobacco plants significantly increased the activity of the promoters of , , and . Basing on this information, we proposed that HblMYB19 and HblMYB44 are the regulators of , , and , which are involved in the biosynthesis pathway of natural rubber.
MYB转录因子在植物次生代谢途径的调控中起着至关重要的作用。橡胶树（v）中的乳管在天然橡胶生产中至关重要，因为天然橡胶在这些结构中形成并储存。为了了解MYB转录因子在这些特化细胞中的作用，我们利用之前获得的橡胶树乳管细胞转录组数据库和公开的橡胶树基因组数据库，鉴定出44个MYB基因（命名为 至 ）。表达谱显示，5个MYB基因在乳管中高表达。选择 和 进行进一步研究。HblMYB19和HblMYB44在酵母中与 、 和 的启动子结合。此外，HblMYB19和HblMYB44在烟草植株中的瞬时过表达显著提高了 、 和 的启动子活性。基于这些信息，我们提出HblMYB19和HblMYB44是 、 和 的调节因子，它们参与天然橡胶的生物合成途径。
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