大鼠胎盘组织体外产生1,25 - 二羟基维生素D3
In vitro production of 1,25-dihydroxyvitamin D3 by rat placental tissue.
作者信息
Tanaka Y, Halloran B, Schnoes H K, DeLuca H F
出版信息
Proc Natl Acad Sci U S A. 1979 Oct;76(10):5033-5. doi: 10.1073/pnas.76.10.5033.
Abstract
Weanling female rats were fed a vitamin D-deficient diet for 4 months until they reached maturity. They were mated with normal, vitamin D-replete male rats and, at 20 days of pregnancy, the female rats were killed and their placentae were removed, homogenzied, and incubated with 25-hydroxyvitamin D3. The incubation mixtures were extracted and the extracts were subjected to Sephadex LH-20 column chromatography followed by high-pressure liquid chromatography. The 1,25-dihydroxyvitamin D3 region of the high-pressure liquid chromatogram was recycled to purity and the structure of the product was identified as 1,25-dihydroxyvitamin D3 by ultraviolet absorption spectrophotometry and by mass spectrometry. Thus it is now evident that placenta, in addition to renal tissue, is capable of converting 25-hydroxyvitamin D3 to the hormonal form of vitamin D, 1,25-dihydroxyvitamin D3.
摘要
将断乳的雌性大鼠喂以缺乏维生素D的饲料4个月，直至其成熟。将它们与正常的、维生素D充足的雄性大鼠交配，在怀孕20天时，处死雌性大鼠并取出其胎盘，匀浆后与25-羟基维生素D3一起孵育。孵育混合物进行提取，提取物先经Sephadex LH-20柱色谱，然后进行高压液相色谱分析。高压液相色谱图中1,25-二羟基维生素D3区域经循环提纯，产物结构通过紫外吸收分光光度法和质谱法鉴定为1,25-二羟基维生素D3。因此，现在很明显，胎盘除了肾脏组织外，也能够将25-羟基维生素D3转化为维生素D的激素形式，即1,25-二羟基维生素D3。
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