湖水、叶浸提液和泥炭土水中溶解有机碳和氮的数量和组成的储存效应。
Storage effects on quantity and composition of dissolved organic carbon and nitrogen of lake water, leaf leachate and peat soil water.
机构信息
Department of Chemical Analytics and Biogeochemistry, Leibniz-Institute of Freshwater Ecology and Inland Fisheries (IGB), Müggelseedamm 301, 12587 Berlin, Germany; Institute for Ecological Chemistry, Plant Analysis and Stored Product Protection, Julius Kühn-Institut, Federal Research Centre for Cultivated Plants, Königin-Luise-Str. 19, 14195 Berlin, Germany.
Department of Chemical Analytics and Biogeochemistry, Leibniz-Institute of Freshwater Ecology and Inland Fisheries (IGB), Müggelseedamm 301, 12587 Berlin, Germany; Faculty of Agriculture and Environment, Landscape Ecology and Site Evaluation, University of Rostock, Justus-von-Liebig-Weg 6, 18059 Rostock, Germany; Department of Bioscience, Aarhus University, Vejlsøvej 25, 8600 Silkeborg, Denmark.
出版信息
Water Res. 2018 Mar 1;130:98-104. doi: 10.1016/j.watres.2017.11.053. Epub 2017 Nov 27.
This study aimed to evaluate the effects of freezing and cold storage at 4 °C on bulk dissolved organic carbon (DOC) and nitrogen (DON) concentration and SEC fractions determined with size exclusion chromatography (SEC), as well as on spectral properties of dissolved organic matter (DOM) analyzed with fluorescence spectroscopy. In order to account for differences in DOM composition and source we analyzed storage effects for three different sample types, including a lake water sample representing freshwater DOM, a leaf litter leachate of Phragmites australis representing a terrestrial, 'fresh' DOM source and peatland porewater samples. According to our findings one week of cold storage can bias DOC and DON determination. Overall, the determination of DOC and DON concentration with SEC analysis for all three sample types were little susceptible to alterations due to freezing. The findings derived for the sampling locations investigated here may not apply for other sampling locations and/or sample types. However, DOC size fractions and DON concentration of formerly frozen samples should be interpreted with caution when sample concentrations are high. Alteration of some optical properties (HIX and SUVA) due to freezing were evident, and therefore we recommend immediate analysis of samples for spectral analysis.
本研究旨在评估冷冻和 4°C 冷藏对批量溶解有机碳（DOC）和氮（DON）浓度以及通过尺寸排阻色谱（SEC）测定的 SEC 级分的影响，以及通过荧光光谱分析对溶解有机物（DOM）的光谱性质的影响。为了说明 DOM 组成和来源的差异，我们分析了三种不同样品类型的储存效应，包括代表淡水 DOM 的湖水样品、代表陆地“新鲜”DOM 来源的芦苇叶浸出液和泥炭地孔隙水样品。根据我们的发现，一周的冷藏会影响 DOC 和 DON 的测定。总体而言，对于所有三种样品类型，通过 SEC 分析测定 DOC 和 DON 浓度很少受到冷冻的影响。此处调查的采样地点得出的结果可能不适用于其他采样地点和/或样品类型。然而，当样品浓度较高时，应谨慎解释先前冷冻样品的 DOC 分子量级分和 DON 浓度。冷冻导致一些光学性质（HIX 和 SUVA）发生变化，因此我们建议立即对样品进行光谱分析。
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