胡椒碱通过AMPK依赖的Runx2表达诱导成骨细胞分化。
Piperine induces osteoblast differentiation through AMPK-dependent Runx2 expression.
作者信息
Kim Do-Young, Kim Eun-Jung, Jang Won-Gu
机构信息
Department of Biotechnology, College of Engineering, Daegu University, Gyeongbuk, 38453, Republic of Korea; Research Institute of Anti-Aging, Daegu University, Gyeongbuk, 38453, Republic of Korea.
Research Institute of Anti-Aging, Daegu University, Gyeongbuk, 38453, Republic of Korea; Department of Immunology, Kyungpook National University School of Medicine, Daegu, 41944, Republic of Korea.
出版信息
Biochem Biophys Res Commun. 2018 Jan 1;495(1):1497-1502. doi: 10.1016/j.bbrc.2017.11.200. Epub 2017 Dec 5.
Piperine is an alkaloid responsible for the pungency of black pepper and long pepper. It is reported to have various biological actions such as anti-oxidative and anti-inflammatory, and aids cancer prevention. Antioxidants have been shown to promote osteoblast differentiation. However, osteoblast differentiation by piperine has not yet been elucidated. Piperine-induced expression of the osteogenic genes such as distal-less homeobox 5 (Dlx5), inhibitor of DNA binding-1 (Id1), and runt-related transcription factor 2 (Runx2) was investigated using RT-PCR. In addition, alkaline phosphatase (ALP) activity and mineralization was found to be increased by piperine treatment. Finally, we confirmed that piperine induced phosphorylation of AMPK in MC3T3-E1 cells. Taken together, these results demonstrate that piperine enhance osteoblast differentiation through AMPK phosphorylation in MC3T3-E1 cells.
胡椒碱是一种生物碱，赋予黑胡椒和荜拨辛辣味。据报道，它具有多种生物学作用，如抗氧化和抗炎作用，还有助于预防癌症。抗氧化剂已被证明可促进成骨细胞分化。然而，胡椒碱对成骨细胞分化的影响尚未阐明。使用逆转录聚合酶链反应（RT-PCR）研究了胡椒碱诱导的远端同源盒5（Dlx5）、DNA结合抑制因子1（Id1）和 runt相关转录因子2（Runx2）等成骨基因的表达。此外，发现用胡椒碱处理可增加碱性磷酸酶（ALP）活性和矿化作用。最后，我们证实胡椒碱可诱导MC3T3-E1细胞中AMPK的磷酸化。综上所述，这些结果表明胡椒碱通过MC3T3-E1细胞中AMPK的磷酸化增强成骨细胞分化。
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