大鼠肝脏溶酶体膜中编码107 kDa唾液糖蛋白的cDNA克隆的分离与测序。

Isolation and sequencing of a cDNA clone encoding 107 kDa sialoglycoprotein in rat liver lysosomal membranes.

作者信息

Himeno M, Noguchi Y, Sasaki H, Tanaka Y, Furuno K, Kono A, Sakaki Y, Kato K

机构信息

Division of Physiological Chemistry, Faculty of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan.

出版信息

FEBS Lett. 1989 Feb 27;244(2):351-6. doi: 10.1016/0014-5793(89)80561-7.

DOI:10.1016/0014-5793(89)80561-7

PMID:2920835

Abstract

A cDNA for 107 kDa sialoglycoprotein (LGP 107), the major protein component of rat liver lysosomal membranes, was isolated and sequenced. The 1.8 kbp cDNA contained an open reading frame encoding a polypeptide consisting of 386 amino acid residues (Mr 41,914). The deduced NH2-terminal 10-residue sequence is identical with that determined for purified LGP 107. The primary structure deduced for LGP 107 contains 20 potential N-glycosylation sites and exhibits 82.5, 43 and 60% sequence similarities to mouse LAMP-1, chicken LEP 100, and a 120-kDa human lysosomal glycoprotein, respectively. Among these lysosomal glycoproteins, the amino acid sequence of the putative transmembrane segment is highly conserved. Northern blot hybridization analysis identified a single species of LGP 107 mRNA (2.1 kbp in length) in rat liver, kidney, brain, lung, spleen, heart and pancreas, although its level in pancreas was very low.

摘要

分离并测序了大鼠肝脏溶酶体膜主要蛋白质成分107kDa唾液酸糖蛋白（LGP 107）的cDNA。该1.8kbp的cDNA包含一个开放阅读框，编码一个由386个氨基酸残基组成的多肽（Mr 41,914）。推导的NH2末端10个残基的序列与纯化的LGP 107测定的序列相同。推导的LGP 107一级结构包含20个潜在的N-糖基化位点，与小鼠LAMP-1、鸡LEP 100和一种120kDa的人溶酶体糖蛋白的序列相似性分别为82.5%、43%和60%。在这些溶酶体糖蛋白中，假定跨膜区的氨基酸序列高度保守。Northern印迹杂交分析在大鼠肝脏、肾脏、大脑、肺、脾脏、心脏和胰腺中鉴定出单一的LGP 107 mRNA（长度为2.1kbp），尽管其在胰腺中的水平非常低。