Hubei International Scientific and Technological Cooperation Base of Waste Conversion by Insects, Huazhong Agricultural University, Wuhan, People's Republic of China.
Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, People's Republic of China.
Environ Sci Pollut Res Int. 2018 Feb;25(6):5379-5385. doi: 10.1007/s11356-017-0856-7. Epub 2017 Dec 5.
Black soldier fly, Hermetia illucens (Linnaeus, 1758), is an important economic fly as its larvae can be used for recycling organic waste, such as food waste and manure. H. illucens larvae (BSFL) could uptake Cd from substrates and accumulate it inside bodies, which need to be monitored during waste treatment. Metallothionein (MT) usually serve as biomarker because of its role in metal homeostasis, detoxification, and dose response of heavy metals. Therefore, a MT gene was cloned from H. illucens (HIMT) that encoded 40 amino acids with typical cysteine rich features, which had a high sequence identity with other insect MTs. The expression of HIMT and total MT protein was measured in BSFL fed by meals spiked with gradient dose of Cd (0, 5, 50, 500 mg/kg) for 24, 48, 72, and 96 h, respectively. Dose-associated response of HIMT and total MT were found and the possible correlative range of Cd was from 5 to 50 mg/kg. The expression of HIMT might be a potential biomarker for monitoring Cd contamination by H. illucens in terrestrial organic matters, which might further apply in waste transformation system.
黑水虻（Hermetia illucens）（Linnaeus，1758）是一种重要的经济昆虫，因为其幼虫可以用来回收有机废物，如食物垃圾和粪便。H. illucens 幼虫（BSFL）可以从基质中摄取 Cd 并在体内积累，这在废物处理过程中需要进行监测。金属硫蛋白（MT）通常作为生物标志物，因为它在金属内稳态、解毒和重金属剂量反应中起作用。因此，从 H. illucens 中克隆了一个 MT 基因（HIMT），该基因编码 40 个氨基酸，具有典型的富含半胱氨酸的特征，与其他昆虫 MTs 具有很高的序列同一性。分别用梯度剂量（0、5、50、500mg/kg）的 Cd 处理 24、48、72 和 96 小时后，测量了 BSFL 中 HIMT 和总 MT 蛋白的表达。发现了 HIMT 和总 MT 的剂量相关反应，Cd 的可能相关范围为 5 至 50mg/kg。HIMT 的表达可能是监测陆地有机物质中 Cd 污染的潜在生物标志物，这可能进一步应用于废物转化系统。
