光合生物中SET结构域蛋白的系统发育关系和结构域组织
Phylogenetic relationship and domain organisation of SET domain proteins of Archaeplastida.
作者信息
Sarma Supriya, Lodha Mukesh
机构信息
Centre for Cellular and Molecular Biology (CSIR), Uppal Road, Habsiguda, Hyderabad, 500007, India.
出版信息
BMC Plant Biol. 2017 Dec 11;17(1):238. doi: 10.1186/s12870-017-1177-1.
BACKGROUND
SET is a conserved protein domain with methyltransferase activity. Several genome and transcriptome data in plant lineage (Archaeplastida) are available but status of SET domain proteins in most of the plant lineage is not comprehensively analysed.
RESULTS
In this study phylogeny and domain organisation of 506 computationally identified SET domain proteins from 16 members of plant lineage (Archaeplastida) are presented. SET domain proteins of rice and Arabidopsis are used as references. This analysis revealed conserved as well as unique features of SET domain proteins in Archaeplastida. SET domain proteins of plant lineage can be categorised into five classes- E(z), Ash, Trx, Su(var) and Orphan. Orphan class of SET proteins contain unique domains predominantly in early Archaeplastida. Contrary to previous study, this study shows first appearance of several domains like SRA on SET domain proteins in chlorophyta instead of bryophyta.
CONCLUSION
The present study is a framework to experimentally characterize SET domain proteins in plant lineage.
背景
SET是一种具有甲基转移酶活性的保守蛋白结构域。植物谱系（古质体生物）中有多个基因组和转录组数据可用，但大多数植物谱系中SET结构域蛋白的状况尚未得到全面分析。
结果
本研究展示了从植物谱系（古质体生物）的16个成员中通过计算鉴定出的506个SET结构域蛋白的系统发育和结构域组织。以水稻和拟南芥的SET结构域蛋白作为参考。该分析揭示了古质体生物中SET结构域蛋白的保守特征和独特特征。植物谱系的SET结构域蛋白可分为五类——E(z)、Ash、Trx、Su(var)和孤儿类。孤儿类SET蛋白主要在早期古质体生物中含有独特结构域。与先前的研究相反，本研究表明，几个结构域如SRA首次出现在绿藻门的SET结构域蛋白上，而非苔藓植物门。
结论
本研究是对植物谱系中SET结构域蛋白进行实验表征的一个框架。
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