Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Cairo, Egypt.
J Appl Microbiol. 2018 Mar;124(3):691-707. doi: 10.1111/jam.13665. Epub 2018 Feb 11.
This work aimed to study, isolate, characterize and stabilize the biosurfactant isolated from actinomycetes found in petroleum contaminated soil.
Optimized production of the biosurfactant from Streptomyces griseoplanus NRRL-ISP5009, SM1 was obtained on day 6 at 30°C, pH 7, 150 rev min , in glycerol yeast extract broth medium supplemented with cellulose, yeast extract and 1% NaCl. The stability of the biosurfactant produced was studied at different temperatures, pH and different concentrations of NaCl. The produced biosurfactant was extracted and purified.
Streptomyces griseoplanus NRRL-ISP5009, SM1 isolated from oil contaminated soil produced a biosurfactant exhibiting emulsification activity. The produced biosurfactant is a mixture of carbohydrate, lipid and protein. It has promising characteristics, including a higher stability at alkaline pH than at acidic pH, a salinity of 1-3% and stable in the temperature range from 0 and 100°C. Also, the potential antimicrobial activity of the purified biosurfactant was recorded.
The research was focused on the isolation of a novel source of biosurfactants that have great importance in the manufacture of food, detergent, pharmaceutical and cosmetics.
本研究旨在从石油污染土壤中发现的放线菌中分离、鉴定和稳定生物表面活性剂。
在 30°C、pH7、150rpm 的条件下，在添加纤维素、酵母提取物和 1%NaCl 的甘油酵母提取物培养基中，于第 6 天，从链霉菌灰色亚种 NRRL-ISP5009 SM1 中优化了生物表面活性剂的生产。研究了不同温度、pH 和不同 NaCl 浓度下产生的生物表面活性剂的稳定性。提取并纯化了产生的生物表面活性剂。
从石油污染土壤中分离出的灰色链霉菌 NRRL-ISP5009 SM1 产生了一种具有乳化活性的生物表面活性剂。产生的生物表面活性剂是碳水化合物、脂质和蛋白质的混合物。它具有良好的特性，包括在碱性 pH 值下比在酸性 pH 值下更稳定、盐度为 1-3%以及在 0 至 100°C 的温度范围内稳定。此外，还记录了纯化生物表面活性剂的潜在抗菌活性。
本研究的重点是分离新型生物表面活性剂的来源，这些生物表面活性剂在食品、洗涤剂、制药和化妆品制造中具有重要意义。
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