胎儿唐氏综合征中母体血浆血管生成和炎症因子分析
Maternal plasma angiogenic and inflammatory factor profiling in foetal Down syndrome.
作者信息
Zbucka-Kretowska Monika, Charkiewicz Karol, Goscik Joanna, Wolczynski Slawomir, Laudanski Piotr
机构信息
Department of Reproduction and Gynecological Endocrinology, Medical University of Bialystok, Bialystok, Poland.
Department of Perinatology and Obstetrics, Medical University of Bialystok, Bialystok, Poland.
出版信息
PLoS One. 2017 Dec 15;12(12):e0189762. doi: 10.1371/journal.pone.0189762. eCollection 2017.
OBJECTIVE AND DESIGN
Angiogenic factors are proteins that are related to certain foetal chromosomal abnormalities. The aim of this study was to determine the concentration of 60 angiogenic factors in the plasma of women with offspring possessing trisomy 21/Down syndrome (DS).
METHOD
After analysing karyotyping results, we selected 20 patients with foetuses possessing DS, and for the control group, we selected 28 healthy patients with uncomplicated pregnancies who delivered healthy newborns at term (i.e., 15-18 weeks of gestation). To assess the concentration of proteins in the blood plasma, we used a protein macroarray which enabled simultaneous determination of 60 angiogenic factors per sample.
RESULTS
We observed a statistically significant increase in the concentration of these five angiogenic and inflammatory factors: TGFb1 (p = 0.039), angiostatin (p = 0.0142), I-309 (p = 0.0476), TGFb3 (p = 0.0395), and VEGF-D (p = 0.0173)-compared to concentrations in patients with healthy foetuses.
CONCLUSION
Our findings suggest that angiogenic factors may play role in DS pathogenesis.
目的与设计
血管生成因子是与某些胎儿染色体异常相关的蛋白质。本研究的目的是测定怀有21三体综合征/唐氏综合征（DS）患儿的女性血浆中60种血管生成因子的浓度。
方法
在分析染色体核型结果后，我们选择了20例怀有DS胎儿的患者，对照组则选择了28例妊娠情况正常且足月分娩健康新生儿的健康患者（即妊娠15 - 18周）。为了评估血浆中蛋白质的浓度，我们使用了一种蛋白质芯片，该芯片能够同时测定每个样本中的60种血管生成因子。
结果
与怀有健康胎儿的患者相比，我们观察到这五种血管生成和炎症因子的浓度有统计学意义的升高：转化生长因子β1（TGFb1，p = 0.039）、血管抑素（p = 0.0142）、I - 309（p = 0.0476）、转化生长因子β3（TGFb3，p = 0.0395）和血管内皮生长因子D（VEGF - D，p = 0.0173）。
结论
我们的研究结果表明血管生成因子可能在DS发病机制中起作用。
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