阿仑膦酸盐对辐照致骨质疏松大鼠骨微结构的影响：显微CT与组织形态计量学分析

Effect of Alendronate on Bone Microarchitecture in Irradiated Rats With Osteoporosis: Micro-CT and Histomorphometric Analysis.

作者信息

Gomes Carolina Cintra, Freitas Deborah Queiroz, Medeiros Araújo Amanda Maria, Ramírez-Sotelo Laura Ricardina, Yamamoto-Silva Fernanda Paula, de Freitas Silva Brunno Santos, de Melo Távora Débora, Almeida Solange Maria

机构信息

Professor, Division of Oral Radiology, Department of Oral Diagnosis, School of Dentistry, University of Anápolis, Anápolis, Brazil.

Professor, Division of Oral Radiology, Department of Oral Diagnosis, Piracicaba Dental School, State University of Campinas, Piracicaba, Brazil.

出版信息

J Oral Maxillofac Surg. 2018 May;76(5):972-981. doi: 10.1016/j.joms.2017.11.019. Epub 2017 Nov 23.

DOI:10.1016/j.joms.2017.11.019

PMID:29247623

Abstract

PURPOSE

The aim of the present study was to evaluate the effect of alendronate (ALN) on the bone microarchitecture of irradiated rats with estrogen deficiency, using microcomputed tomography (micro-CT) and histomorphometric analysis.

MATERIALS AND METHODS

Forty adult Wistar rats were subjected to ovariectomy and randomly divided into the following groups: control (CON), ALN, irradiated (IRR), and ALN/irradiated (ALN/IRR). Approximately 50 days after ovariectomy, the hind limbs of the rats in the IRR and ALN/IRR groups were irradiated with 15 Gy of x-radiation. The rats were euthanized 7 and 30 days after irradiation. The bone microarchitecture was analyzed using micro-CT and histomorphometry. The bone microarchitecture was evaluated using the Mann-Whitney U test, analysis of variance, and the post hoc Tukey test, with statistical significance set at 5%.

RESULTS

Irradiation had increased the thickness of the cortical bone at 7 days (P < .05) and also decreased the number of trabeculae per unit length and increased the average distance between the trabeculae (P < .05) at 30 days. ALN inhibited the deleterious effect of x-radiation, preventing the distance between the trabeculae from increasing and the number of trabeculae per unit length from decreasing (P < .05).

CONCLUSIONS

The present results have demonstrated that the initial effect of ALN could be positive, because it checked the deleterious action in the bone tissue submitted to x-radiation.

摘要

目的

本研究旨在使用微型计算机断层扫描（micro-CT）和组织形态计量学分析，评估阿仑膦酸盐（ALN）对雌激素缺乏的辐照大鼠骨微结构的影响。

材料与方法

40只成年Wistar大鼠接受卵巢切除术，并随机分为以下几组：对照组（CON）、ALN组、辐照组（IRR）和ALN/辐照组（ALN/IRR）。卵巢切除术后约50天，对IRR组和ALN/IRR组大鼠的后肢进行15 Gy的X射线辐照。辐照后7天和30天对大鼠实施安乐死。使用micro-CT和组织形态计量学分析骨微结构。采用Mann-Whitney U检验、方差分析和事后Tukey检验评估骨微结构，设定统计学显著性水平为5%。