针对变性DNA产生的抗体可与双链DNA结合。

Antibodies raised against denatured DNA bind to double-stranded DNA.

作者信息

Vaishnav Y N, Antony A

机构信息

Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore.

出版信息

J Immunol Methods. 1989 Mar 10;118(1):25-30. doi: 10.1016/0022-1759(89)90048-3.

DOI:10.1016/0022-1759(89)90048-3

PMID:2926149

Abstract

Antibodies raised against denatured DNA complexed with methylated bovine serum albumin have been reported to react with ssDNA but not with dsDNA. Using a highly sensitive avidin-biotin microELISA, we report that such antibodies also bind to dsDNA. Antibodies which reacted with ssDNA and dsDNA were found to be of IgG type. The antibodies did not react with tRNA and rRNA. The binding of antibodies to dsDNA was partially inhibited by individual deoxyribonucleotides. ssDNA as well as dsDNA inhibited the binding of antibodies to dsDNA. The binding of these antibodies to supercoiled and relaxed forms of pBR322 DNA was demonstrated by gel retardation assay. The cross-reaction with ssDNA was observed even after affinity purification on native DNA-cellulose. The antibodies were also shown to bind to poly(dA-dT).poly(dA-dT).

摘要

据报道，针对与甲基化牛血清白蛋白复合的变性DNA产生的抗体可与单链DNA反应，但不与双链DNA反应。我们使用高度灵敏的抗生物素蛋白-生物素微酶联免疫吸附测定法报告称，此类抗体也能与双链DNA结合。发现与单链DNA和双链DNA反应的抗体为IgG型。这些抗体不与tRNA和rRNA反应。单个脱氧核糖核苷酸可部分抑制抗体与双链DNA的结合。单链DNA和双链DNA均抑制抗体与双链DNA的结合。通过凝胶阻滞试验证明了这些抗体与pBR322 DNA的超螺旋形式和松弛形式的结合。即使在天然DNA-纤维素上进行亲和纯化后，仍观察到与单链DNA的交叉反应。还显示这些抗体可与聚（dA-dT）.聚（dA-dT）结合。