具有和不具有α0-地中海贫血特征的纯合子HbE中HbA2、E、F及红细胞参数的比较
Comparison of HbA2, E, F and Red Cell Parameters in Homozygous HbE With and Without α0-Thalassemia Trait.
作者信息
Pornprasert Sakorn, Thichak Somkhid, Kongthai Kanyakan, Wangchauy Chaowanee
机构信息
Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, Thailand.
Regional Medical Sciences Center Chiang Mai, Chiang Mai, Thailand.
出版信息
Lab Med. 2018 Mar 21;49(2):118-122. doi: 10.1093/labmed/lmx083.
OBJECTIVES
To compare levels of HbA2, HbE, HbF, and red cell parameters (total Hb, PCV, MCV, and MCH) and also determine their appropriated cut-off points for initial discrimination between homozygous HbE with and without α0-thalassemia trait.
METHODS
Hb analysis results from capillary electrophoresis (CE) and red cell parameters of homozygous HbE without α0-thalassemia trait (n = 41) and with α0-thalassemia trait (n = 17) were reviewed.
RESULTS
The MCV, MCH, and HbE of homozygous HbE with α0-thalassemia trait were significantly lower than those of homozygous HbE without α0-thalassemia, while HbA2 levels of the former were significantly higher than those of the latter. HbA2 at a cut-off point of 5.3% had 69.0% efficiency in discrimination between the 2 groups. It could also reduce 56.1% of homozygous HbE samples for α0-thalassemia testing.
CONCLUSIONS
The elevated HbA2 ≥5.3% is a useful marker for initial discrimination between homozygous HbE with and without α0-thalassemia trait.
目的
比较HbA2、HbE、HbF水平以及红细胞参数（总血红蛋白、红细胞压积、平均红细胞体积和平均红细胞血红蛋白含量），并确定其合适的截断点，用于初步鉴别有无α0地中海贫血特征的纯合子HbE。
方法
回顾了无α0地中海贫血特征的纯合子HbE（n = 41）和有α0地中海贫血特征的纯合子HbE（n = 17）的毛细管电泳（CE）血红蛋白分析结果及红细胞参数。
结果
有α0地中海贫血特征的纯合子HbE的平均红细胞体积、平均红细胞血红蛋白含量和HbE显著低于无α0地中海贫血特征的纯合子HbE，而前者的HbA2水平显著高于后者。截断点为5.3%时，HbA2鉴别两组的效率为69.0%。它还可以减少56.1%的纯合子HbE样本进行α0地中海贫血检测。
结论
HbA2升高≥5.3%是初步鉴别有无α0地中海贫血特征的纯合子HbE的有用标志物。
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