体外和体内评估蜜橘果皮的全身毒性和遗传毒性。
In vitro and in vivo evaluation of systemic and genetic toxicity of Citrus unshiu peel.
机构信息
KM (Korean Medicine) Application Center, Korea Institute of Oriental Medicine, 70 Cheomdan-ro, Dong-gu, Daegu 41062, South Korea.
KM (Korean Medicine) Application Center, Korea Institute of Oriental Medicine, 70 Cheomdan-ro, Dong-gu, Daegu 41062, South Korea.
出版信息
J Ethnopharmacol. 2018 Apr 6;215:120-123. doi: 10.1016/j.jep.2017.12.029. Epub 2017 Dec 20.
ETHNOPHARMACOLOGICAL RELEVANCE
The peel of Citrus unshiu Markovich fruits (CUP), called "Jinpi" in Korea, and "Chenpi" in China, has been used for the treatment of respiratory and blood circulation disorders in traditional oriental medicine (TOM). Despite its widespread uses in TOM, no information on the safety of CUP has been reported. Thus, genotoxicity and systemic toxicity of CUP were evaluated in the current studies.
MATERIALS AND METHODS
We conducted a toxicological evaluation of CUP water extracts using acute and subchronic (13-week repeated-dose) toxicity tests and three genotoxicity assays (bacterial reverse mutation, mammalian chromosomal aberration, and micronuclei formation).
RESULTS
In acute and subchronic toxicity tests, both the median lethal dose (LD) and no-observed-adverse-effect level (NOAEL) were more than 4000mg/kg/day in rats. None of the genotoxicity assays revealed any mutagenicity or clastogenicity in in vitro and in vivo systems.
CONCLUSION
CUP water extracts were found to be nongenotoxic under our testing conditions and had low acute and subchronic toxicity.
民族药理学相关性
柚子（Citrus unshiu Markovich）的果皮（CUP），在韩国被称为“Jinpi”，在中国被称为“陈皮”，一直被用于传统东方医学（TOM）治疗呼吸和血液循环紊乱。尽管在 TOM 中广泛使用，但没有关于 CUP 安全性的信息报告。因此，在当前研究中对 CUP 的遗传毒性和全身毒性进行了评估。
材料和方法
我们使用急性和亚慢性（13 周重复剂量）毒性试验以及三种遗传毒性试验（细菌回复突变、哺乳动物染色体畸变和微核形成）对 CUP 水提取物进行了毒理学评价。
结果
在急性和亚慢性毒性试验中，大鼠的半数致死剂量（LD）和无观察不良效应水平（NOAEL）均高于 4000mg/kg/天。在体外和体内系统中，没有任何遗传毒性试验显示出致突变性或染色体断裂性。
结论
在我们的测试条件下，CUP 水提取物被发现无遗传毒性，且具有低急性和亚慢性毒性。
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