Du S H, Li D R, Wang H J, Wang Q
School of Forensic Medicine, Southern Medical University, Guangzhou 510515, China.
Fa Yi Xue Za Zhi. 2017 Oct;33(5):526-531. doi: 10.3969/j.issn.1004-5619.2017.05.017. Epub 2017 Oct 25.
Reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) is a convenient and highly efficient method for the detection of mRNA in tissues or body fluid samples. It has the characteristics of easy operation, high sensitivity and specificity, etc. With a wide application in medicine, biology and other fields, RT-qPCR technique has made some progresses in the research field of forensic pathology. This paper reviews the application value of RT-qPCR in the study of forensic pathology and current situation, as well as the research progress at home and abroad reviews. It also summarizes the notes of samples extraction, RT-qPCR experiments and data processing, which aims to provide reference for the forensic research and its application.
逆转录定量实时聚合酶链反应(RT-qPCR)是一种用于检测组织或体液样本中mRNA的便捷且高效的方法。它具有操作简便、灵敏度高和特异性强等特点。RT-qPCR技术在医学、生物学等领域有着广泛应用,在法医病理学研究领域也取得了一些进展。本文综述了RT-qPCR在法医病理学研究中的应用价值、现状以及国内外的研究进展。同时总结了样本提取、RT-qPCR实验及数据处理的注意事项,旨在为法医研究及其应用提供参考。